Napabucasin prevents brain injury in neuronal neonatal rat cells through suppression of apoptosis and inflammation. (March 2019)

• Record Type: Journal Article
• Authors: Wang, Jun; Feng, Hang; Li, Zhe; Zhang, Xiaoge
• Is Part Of: Microbial pathogenesis. Volume 128(2019)
• Page Start: 337
• Page End: 341
• Publication Date: 2019-03
• Subjects: Catalase -- Interleukin -- Neuroprotection -- Apolipoprotein -- Oxidative damage; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2019.01.019
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: The present study investigates the protective effect of napabucasin on the expression of apoptosis markers and inflammatory factors in the neuronal rat cells with post-isolation damage. The level of ROS determined by the fluorescence measurement in the neuronal rat cells with post-isolation damage was 310.21 RFU compared to 21.45 RFU in sham cell cultures. Napabucasin treatment decreased ROS level in the neuronal rat cells with post-isolation damage in dose based manner. ROS level decreased to 278.67, 203.65, 163.32 and 26.87 RFU, respectively in 1, 2, 3 and 4 μM napabucasin treated cell cultures. Treatment with napabucasin increased GSH level significantly (P < 0.05) in the neuronal rat cells with post-isolation damage. Napabucasin treatment at with 1, 2, 3 and 4 μM concentrations increased SOD activity to 2.4, 3.6, 5.1 and 6.1 U/mg, respectively. Treatment with napabucasin increased the activity of catalase in dose based manner. Napabucasin treatment increased Gpx in injured brain cells of neonatal rats. A significant (P < 0.05) increase in the activity of AChE was observed in neuronal rat cells with post-isolation damage on treatment with napabucasin. Treatment with napabucasin reduced the level of TNF-α and IL-6 significantly (P < 0.05) compared to untreated group. Napabucasin treatment decreased the expression of Bax, caspase-3 and p53 proteins in the neuronal rat cells with post-isolation damage. Napabucasin treatment protects post-isolation damage in the neuronal cells of neonatal rats by suppression of apoptosis and oxidative stress. Therefore, napabucasin can be used for the treatment of brain injury. Highlights: Napabucasin decreased ROS level in the neuronal rat cells with post-isolation damage. It increased GSH level in the neuronal rat cells with post-isolation damage. Treatment with napabucasin increased the activity of catalase in dose based manner. It increased Gpx in the neuronal rat cells with post-isolation damage. An increase in AChE activity was observed on treatment with napabucasin. It decreased Bax, caspase-3 and p53 proteins expression. Napabucasin protects post-isolation damage by suppression of apoptosis and oxidative stress.