Xenotransplantation of goat ovary as an alternative to analyse follicles after vitrification. Issue 2 (19th October 2018)

• Record Type: Journal Article
• Authors: Jiatsa Donfack, Nathalie; Alves, Kele Amaral; Alves, Benner Geraldo; Pedrosa Rocha, Rebeca Magalhães; Bruno, Jamily Bezerra; Lobo, Carlos H.; Bertolini, Marcelo; dos Santos, Regiane Rodrigues; Taumaturgo, Marilia de Oliveira; Raposo, Ramon da Silva; de Figueiredo, José Ricardo; Smitz, Johan; Ribeiro Rodrigues, Ana Paula
• Is Part Of: Reproduction in domestic animals. Volume 54:Issue 2(2019)
• Page Start: 216
• Page End: 224
• Publication Date: 2018-10-19
• Subjects: folliculogenesis -- graft -- ovarian cryopreservation -- ovarian function restoration; Animal breeding -- Periodicals; Veterinary pathology -- Periodicals; Livestock -- Breeding -- Periodicals; 636.082
• DOI: 10.1111/rda.13340
• Languages: English
• ISSNs: 0936-6768

Abstract

Contents: The aim of this study was to evaluate the caprine preantral follicles enclosed on vitrified/warmed ovarian cortex grafted to nude BALB/mice during 1 month. The ovarian cortex from goats was fragmented (3 × 3 × 0.5 mm) and divided into four groups: fresh control, vitrified control, fresh transplant and vitrified transplant . Follicular morphology, development and density, fibrosis as well as apoptosis, and tissue revascularization were evaluated. It was also observed a significant decrease in morphologically normal preantral (primordial, transition, primary and secondary) follicles in both vitrified control and vitrified transplant treatments when compared with both fresh control and fresh transplant . However, fresh control and fresh transplant exhibited a similar percentage of developing follicles. Additionally, Vitrified control showed a significant increase in developing follicles in comparison with both fresh control and fresh transplant . Follicular density significantly decreased in all treatments in comparison with fresh control . We observed high fibrosis in both fresh transplant and vitrified transplant . The mRNA expression of caspase 3 was lower in both fresh transplant and vitrified transplant in comparison with vitrified control . In conclusion, xenotransplantation is an excellent strategy to maintain normal preantral follicle morphology after vitrification/warming of goat ovarian tissue. Yet, in order to ensure the survival and development of these follicles, it is essential to improve the revascularization of the graft.