Challenges, progress and promises of metabolite annotation for LC–MS-based metabolomics. (February 2019)

• Record Type: Journal Article
• Authors: Chaleckis, Romanas; Meister, Isabel; Zhang, Pei; Wheelock, Craig E
• Is Part Of: Current opinion in biotechnology. Volume 55(2019)
• Page Start: 44
• Page End: 50
• Publication Date: 2019-02
• Subjects: Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.copbio.2018.07.010
• Languages: English
• ISSNs: 0958-1669

Abstract

Graphical abstract: Highlights: Data independent MS/MS acquisition methods improve metabolite identification. A step-by-step workflow is needed to assess the accuracy of metabolite identification. Community databases compiling spectral and raw data will play an increasing role in metabolite identification. Abstract : Accurate annotation is vital for data interpretation; however, metabolite identification is a major bottleneck in untargeted metabolomics. Although community guidelines for metabolite identification were published over a decade ago, adaptation of the recommended standards has been limited. The complexity of LC–MS data due to combinations of various chromatographic and mass spectrometric acquisition methods has resulted in the advent of diverse workflows, which often involve non-standardized manual curation. Herein, we review the parameters involved in metabolite reporting and provide a workflow to estimate the level of confidence in reported metabolite annotation. The future of metabolite identification will be heavily based upon the use of metabolome data repositories and associated data analysis tools, which will enable data to be shared, re-analyzed and re-annotated in an automated fashion.