Interruption of immune responses in primary macrophages exposed to nonylphenol provides insights into the role of ER and NF-KB in immunotoxicity of Persian sturgeon. Issue 86 (March 2019)

• Record Type: Journal Article
• Authors: Rastgar, Sara; Movahedinia, Abdolali; Salamat, Negin; Salati, Amir Parviz; Zabihi, Ebrahim
• Is Part Of: Fish & shellfish immunology. Issue 86(2019)
• Page Start: 125
• Page End: 134
• Publication Date: 2019-03
• Subjects: Nonylphenol -- Immunotoxic -- Acipencer persicous; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2018.11.038
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The severe decline in population of sturgeons due to pollution highlighted poor understanding about the immunotoxicological responses of sturgeons. This study was designed in three experiments to find out how nonylphenol (NP) interrupts some pro-inflammatory immune parameters in macrophages from Persian sturgeon ( Acipencer persicous ) as the oldest vertebrate model conserving intact innate immune system. After determination of IC50 values of NP (200 μM), some pro-inflammatory immune parameters and induced apoptosis in macrophages at low dose (10 nM) and high dose (100 nM) of NP and of 17β estradiol (E2) (positive control) were determined after 6, 24 and 48 h of the exposure (as the first experiment). The two doses of NP induced pro-inflammatory reaction and apoptosis with strong correlations, whereas this result was observed more obviously in high dose of E2. In the second experiments, the macrophages were exposed to the two doses of NP along with estrogen receptor alpha (ERα) antagonist, which consequently decreased the induction of pro-inflammatory reactions. Similarly, in the third experiment, NF-KB and ERα antagonists were used and pro-inflammatory reactions decreased compared to the control group (P < 0.05). Decreasing correlation between immune parameters following the second and third experiments verified interaction between ERα and NF-KB pathways. Thus, NP could be immune disrupter and apoptosis inducer in sturgeon macrophages in vitro, even in low dose. For the first time, this study revealed that NP can induce pro-inflammatory reactions in macrophages derived from sturgeons. Highlights: Immunotoxicological responses in sturgeon ancient fish to xenoestrogen Nonylphenol interrupts some pro-inflammatory immune parameters in macrophages from Persian sturgeon. Nonylphenol has potential of apoptosis induction in Persian sturgeon macrophages in dose and time dependent manner. Synergistic interaction between ERα and NF-KB pathways after exposure to nonylphenol in Persian sturgeon macrophages.