Antioxidative status, immunological responses, and heat shock protein expression in hepatopancreas of Chinese mitten crab, Eriocheir sinensis under the exposure of glyphosate. Issue 86 (March 2019)

• Record Type: Journal Article
• Authors: Hong, Yuhang; Huang, Yi; Yan, Guangwen; Pan, Chao; Zhang, Jilei
• Is Part Of: Fish & shellfish immunology. Issue 86(2019)
• Page Start: 840
• Page End: 845
• Publication Date: 2019-03
• Subjects: glyphosate -- Eriocheir sinensis -- Oxidative stress -- Immune response -- Heat shock protein; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2018.12.020
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: As a broad-spectrum herbicide, glyphosate was extensively utilised in China for several decades. The contradiction between glyphosate spraying and crab breeding in the rice-crab co-culture system has become more obvious. In this study, the antioxidative status and immunological responses of Chinese mitten crab, Eriocheir sinensis, under sublethal exposure of glyphosate were investigated by detecting the antioxidative and immune-related enzyme activity, acetylcholinesterase (AChE) activity and relative mRNA expression of heat shock proteins (HSPs) in hepatopancreas. The results showed that high concentrations of glyphosate (44 and 98 mg/L) could induce significant alteration of superoxide dismutase (SOD), peroxidase (POD), acid phosphatase (ACP), alkaline phosphatase (AKP), and phenoloxidase (PO) activities by first rising then falling during the exposure. However, AChE activity in all treatments including 4.4 mg/L was inhibited markedly after 6 h of exposure. In addition, the relative mRNA expression of HSP 60, HSP 70, and HSP 90 was significantly upregulated at both 48 h and 96 h. These results revealed that glyphosate has a prominent toxic effect on E. sinensis based on antioxidative and immunological response inhibition and AChE activity reduction even at the lowest concentration of 4.4 mg/L, and a protective response by upregulation of HSPs was carried out by the species to ease the environmental stress. Highlights: The antioxidative and immunological response of Erocheir Sinensis exposed to glyphosate were investigated in this study. Glyphosate could induce significant antioxidative and immune inhibition under sublethal concentration. The AChE activity and HSP 70 expression in hepatopancreas were considered more sensitive parameters.