Determinants of vitamin D receptor gene expression in visceral and subcutaneous adipose tissue in non-obese, obese, and morbidly obese subjects. Issue 187 (March 2019)

• Record Type: Journal Article
• Authors: Yuzbashian, Emad; Asghari, Golaleh; Hedayati, Mehdi; Zarkesh, Maryam; Mirmiran, Parvin; Khalaj, Alireza
• Is Part Of: Journal of steroid biochemistry and molecular biology. Issue 187(2019)
• Page Start: 82
• Page End: 87
• Publication Date: 2019-03
• Subjects: 25(OH)D 25-hydroxyvitamin D -- BMI body mass index -- SBP systolic blood pressure -- DBP diastolic blood pressure -- VDR vitamin D receptor -- HOMA-IR Homeostatic Model Assessment for Insulin Resistance; VDR -- 25(OH)D -- Dietary intake; Steroid hormones -- Periodicals; Biochemistry -- Periodicals; Hormones -- Periodicals; Molecular Biology -- Periodicals; Hormones stéroïdes -- Périodiques; Steroid hormones; Periodicals; 572.579
• DOI: 10.1016/j.jsbmb.2018.11.004
• Languages: English
• ISSNs: 0960-0760

Abstract

Highlights: The determinative factors on VDR gene expression of adipose tissue varied and depended on the degree of obesity. Serum 25(OH)D was the common factor to determine VDR gene expression visceral adipose tissue in all strata of BMI groups. BMI and HOMA-IR were the positive predictors of subcutaneous adipose tissue VDR gene expression. Abstract: We aimed to illustrate determinants of VDR gene expression in visceral and subcutaneous adipose tissue among individuals without diabetes. We gathered visceral and subcutaneous adipose tissues during an elective abdominal surgery form 33 morbidly obese (BMI > 40 kg/m 2 ), 23 obese (BMI = 30–40 kg/m 2 ), and 35 non-obese (BMI<30 kg/m 2 ) participants who were free of diabetes. Participants were classified according to their degree of obesity. Before the surgery, habitual dietary intake, physical activity, 25(OH)D, body mass index (BMI), waist circumference (WC), and HOMA-IR was gathered. Non-obese participants had significantly lower mean VDR gene expression in visceral adipose tissues than both the obese and morbidly obese ones and had also lower expression in subcutaneous adipose tissues than the morbidly obese participants. In multiple linear regression models, BMI and HOMA-IR were the independent positive predictors of VDR gene expression in subcutaneous fat. Among non-obese subjects, WC and 25(OH)D were the positive and negative independent predictors of visceral adipose tissue VDR gene expression, respectively. Among obese participants, 25(OH)D was negatively, and BMI and HOMA-IR were positively associated with VDR mRNA levels in visceral adipose tissue. In morbidly obese participants, the independent positive predictors of VDR gene expression in visceral fat were BMI and HOMA-IR, and negative predictors were 25(OH)D and calcium intake. Our findings suggested that 25(OH)D concentrations are the fundamental elements to determine VDR gene expression in visceral fat which by increasing fat depots, the subsequent insulin resistance became another predictor of VDR gene expression.