Enhanced production of target bioactive metabolites produced by Pseudomonas aeruginosa LV strain. (January 2019)

• Record Type: Journal Article
• Authors: Bedoya, J.C.; Dealis, M.L.; Silva, C.S.; Niekawa, E.T.G.; Navarro, M.O.P.; Simionato, A.S.; Modolon, Fluvio; Chryssafidis, A.L.; Andrade, G.
• Is Part Of: Biocatalysis and agricultural biotechnology. Number 17(2019)
• Page Start: 545
• Page End: 556
• Publication Date: 2019-01
• Subjects: Optimization -- Response surface methodology -- Bioprocess -- Chromatography; Agricultural biotechnology -- Periodicals; Enzymes -- Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.bcab.2018.12.024
• Languages: English
• ISSNs: 1878-8181

Abstract

Abstract: Pseudomonas aeruginosa LV strain produces phenazine-1-carboxylic acid – PCA, phenazine-1-carboxamide – PCN, 2-carboxi-2-heptano-indol-3-ona – IDC, and fluopsin C – OAC. These molecules from secondary metabolism showed a potential for many biotechnological applications. The concentration of every compound obtained in the culture medium was below 6 mg L −1 and, in order to increase these concentrations a process of statistical optimization was carried out. Under optimum conditions the production of IDC, PCA, PCN and OAC increased 42, 38, 250 and 0.84 folds, respectively. The minimum inhibitory concentration of OAC against P. aeruginosa LV strain was 15 mg L −1, indicating that the concentration obtained (11.11 ± 1.05 mg L −1 ) was very close to the maximum concentration possible in the medium. The optimized conditions presented here enhance the production of IDC, PCA, PCN and OAC by P. aeruginosa LV strain and should be implemented in this bacterial culture. Highlights: The LV strain produces phenazines, 2-carboxi-2-heptano-indol-3-ona and fluopsin C. The production of metabolites by LV strain was optimized by RSM methodology. RSM methodology improved the yields and may reduce the cost of production. The fluopsin C inhibited the growth of LV strain at 15 µg mL −1 .