Isolation, purification and identification of the active compound of turmeric and its potential application to control cucumber powdery mildew. (17th May 2018)

• Record Type: Journal Article
• Authors: Fu, W. J.; Liu, J.; Zhang, M.; Li, J. Q.; Hu, J. F.; Xu, L. R.; Dai, G. H.
• Is Part Of: Journal of agricultural science. Volume 156:Number 3(2018)
• Page Start: 358
• Page End: 366
• Publication Date: 2018-05-17
• Subjects: (+)-(S)-ar-turmerone, -- biopesticide, -- cucumber powdery mildew, -- turmeric; Agriculture -- Periodicals; 630.5
• DOI: 10.1017/S0021859618000345
• Languages: English
• ISSNs: 0021-8596

Abstract

Abstract: Cucumber powdery mildew is a destructive foliar disease caused by Podosphaera xanthii (formerly known as Sphaerotheca fuliginea ) that substantially damages the yield and quality of crops. The control of this disease primarily involves the use of chemical pesticides that cause serious environmental problems. Currently, numerous studies have indicated that some plant extracts or products potentially have the ability to act as natural pesticides to control plant diseases. It has been reported that turmeric ( Curcuma longa L.) and its extract can be used in agriculture due to their insecticidal and fungicidal properties. However, the most effective fungicidal component of this plant is still unknown. In the current study, the crude extract of C. longa L. was found to have a fungicidal effect against P. xanthii. Afterwards, eight fractions (Fr.1–Fr.8) were gradually separated from the crude extract by column chromatography. Fraction 1 had the highest fungicidal effect against this pathogen among the eight fractions. The active compound, (+)-( S )- ar -turmerone, was separated from Fr 1 by semi-preparative high-performance liquid chromatography and identified based on its 1 H nuclear magnetic resonance (NMR) and 13 C NMR spectrum data. The EC50 value of (+)-( S )- ar -turmerone was found to be 28.7 µg/ml. The compound also proved to have a curative effect. This is the first study to report that the compound (+)-( S )- ar -turmerone has an effect on controlling this disease. These results provide a basis for developing a new phytochemical fungicide from C. longa L. extract.