Pt(II)‐Based Artificial Nitroreductase: An Efficient and Highly Stable Nanozyme. Issue 4 (28th January 2019)

• Record Type: Journal Article
• Authors: Aghahosseini, Hamideh; Tabatabaei Rezaei, Seyed Jamal; Maleki, Mahshid; Abdolahnjadian, Davod; Ramazani, Ali; Shahroosvand, Hashem
• Is Part Of: ChemistrySelect. Volume 4:Issue 4(2019)
• Page Start: 1387
• Page End: 1393
• Publication Date: 2019-01-28
• Subjects: Pt(II)-based artificial nitroreductase -- Nanozyme -- Nitrophenol reduction -- Experimental design -- Michaelis-Menten kinetics; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.201803776
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: The nitroreductases are a family of natural enzymes with great importance. Inspired the integration of biology and nanotechnology, herein, a rational design for the preparation of the platinum(II)‐based artificial nitroreductase is reported. Engineering the individual ligand scaffold, which on the one hand could form a highly stable complex with the platinum(II) as the catalytic active site and on the other hand could provide halogen bonding site for induce catalysis by approximation, creates an efficient and long‐term resistance artificial nitroreductase. The platinum(II)‐based artificial nitroreductase was characterized by FTIR, TEM, XRD, EDAX and ICP techniques. The reaction conditions were fully optimized for the reduction of high concentration p ‐nitrophenol as a specific substrate in aqueous solution employing experimental design, which highlights the potential application of the designed artificial nitroreductase in wastewater treatment. Moreover, the enzyme‐like Michaelis‐Menten kinetics of the reaction was investigated. The prepared platinum(II)‐based artificial nitroreductase could be stable even after 20 catalytic cycles with excellent activity and it was represented a good turnover frequency. Abstract : An efficient and highly stable Pt(II)‐based artificial nanozyme with enzyme‐like nitroreductase activity was developed. The synergic effect of the highly stable platinum(II) complex as the catalytic active site which could be stable even after 20 catalytic cycles, halogenated ligand as a substrate binding site and magnetite nanoparticles core that could function as a protein mimic, could endow this platinum(II)‐based artificial nitroreductase all the necessary characteristics of an artificial enzyme.