Application of Isothermal Nucleic Acid Signal Amplification in the Detection of Hepatocellular Carcinoma‐Associated MicroRNA. Issue 1 (5th October 2018)

• Record Type: Journal Article
• Authors: Mao, Dongsheng; Chen, Hong; Tang, Yingying; Li, Jingwen; Cao, Ya; Zhao, Jing
• Is Part Of: ChemPlusChem. Volume 84:Issue 1(2019)
• Page Start: 8
• Page End: 17
• Publication Date: 2018-10-05
• Subjects: cancer -- isothermal nucleic acid signal amplification -- microRNA -- RNA recognition -- tumor markers; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/cplu.201800382
• Languages: English
• ISSNs: 2192-6506

Abstract

Abstract: Hepatocellular carcinoma (HCC) is a common type of malignant tumor with high incidence and mortality, and is the third leading cause of cancer‐related death in human. High‐precision detection of tumor markers, such as microRNA (miRNA), is expected to contribute to the early diagnosis of HCC as well as improve the survival rate of HCC patients. In recent years, great effort has been made to develop different methods to detect HCC‐associated miRNA, in which signal amplification strategies are incorporated to realize high sensitivity. Among them, isothermal nucleic acid signal amplification is an emerging signal amplification technology which has the advantages of high amplification efficiency, good reproducibility, and easy operation for real‐time analysis. Herein, we provide an overview of the advances in the application of isothermal nucleic acid signal amplification in HCC‐associated miRNA detection by highlighting several representative works in recent years. Abstract : MicroRNA is a low‐abundance biomarker closely linked to the occurrence and development of hepatocellular carcinoma. Isothermal nucleic acid signal amplification is an efficient and sensitive microRNA detection method that is easy to conduct without extensive instrumentation. The application of this method for the detection of microRNA associated with hepatocellular carcinoma is reviewed.