Dietary supplementation of vitamin C: an effective measure for protection against UV-B irradiation using fish as a model organism. Issue 1 (16th November 2018)

• Record Type: Journal Article
• Authors: Chakrabarti, R.; Singh, M. K.; Sharma, J. G.; Mittal, P.
• Is Part Of: Photochemical & photobiological sciences. Volume 18:Issue 1(2019)
• Page Start: 224
• Page End: 231
• Publication Date: 2018-11-16
• Subjects: Photochemistry -- Periodicals; Photobiology -- Periodicals; 541.35
• DOI: 10.1039/c8pp00481a
• Languages: English
• ISSNs: 1474-905X

Abstract

Abstract : The development of UV-B protective mechanisms in aquacultural species is essential for the sustainable production of healthy aqua crop. Abstract : The development of UV-B protective mechanisms in aquacultural species is essential for the sustainable production of healthy aqua crop. Freshwater carp Catla catla larvae (13.5 ± 1.12 mg) were fed with a diet containing 0.5% vitamin C (D1) and a control diet (D2) for 40 days. Each group was exposed to two doses of UV-B irradiation: 360 (5 min, D15 min and D25 min ) and 720 mJ cm −2 (10 min, D110 min and D210 min ) for 15 days. Significantly ( p < 0.05) higher survival and average weight were recorded in D1 compared to D2 exposed to the same dose. Also, significantly ( p < 0.001) higher nitric oxide synthase and lower thiobarbituric acid reactive substances and heat shock protein 70 levels were recorded in D15 min compared to the other groups. A direct relationship was found between the dose of UV-B and DNA fragmentation in muscles. DNA damage indices such as tail DNA, tail extent moment and olive tail moment were significantly ( p < 0.01) lower in D15 min . Thus, supplementation of vitamin C in the diet provides UV-B protection to larvae.