A label-free immunosensor for the detection of nuclear matrix protein-22 based on a chrysanthemum-like Co-MOFs/CuAu NWs nanocomposite. Issue 2 (27th November 2018)

• Record Type: Journal Article
• Authors: Li, Siyuan; Yue, Song; Yu, Chao; Chen, Yiyu; Yuan, Dong; Yu, Qiubo
• Is Part Of: Analyst. Volume 144:Issue 2(2019)
• Page Start: 649
• Page End: 655
• Publication Date: 2018-11-27
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c8an01590b
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : In this study, a new, simple, and label-free electrochemical immunosensor was presented for the detection of nuclear matrix protein-22 (NMP-22). Abstract : In this study, a new, simple, and label-free electrochemical immunosensor was presented for the detection of nuclear matrix protein-22 (NMP-22). In order to accurately monitor very small amounts of NMP-22, it was advantageous to use highly efficient nanomaterials as signals. For this reason, we synthesized a chrysanthemum-like nanocomposite (Co-MOFs/CuAu NWs), using Co-based metal–organic frameworks (Co-MOFs) as carriers and copper gold nanowires (CuAu NWs) wrapped around their surface, which was applied for modifying a glassy carbon electrode (GCE). The Co-MOFs/CuAu NWs possessed outstanding catalytic capabilities, which served as signal materials and simultaneously carried the anti-NMP-22 antibody (Ab). When different concentrations of the NMP-22 antigen (Ag) were specifically attached to the immunosensor, the current responses decreased by varying degrees. The designed biosensor used the principle to establish a linear regression equation and achieve an accurate quantification of NMP-22. After optimization, the NMP-22 sensor exhibited a good linear response over a concentration range from 0.1 pg mL −1 to 1 ng mL −1, with a lower detection limit of 33 fg mL −1 (based on S/N = 3). The proposed biosensor demonstrated the advantages of ultra-sensitivity, high specificity and acceptable reproducibility, suggesting that the proposed strategy has the potential for the quantification of NMP-22 in human urine samples. Moreover, the novel nanocomposite Co-MOFs/CuAu NWs are promising materials for electrochemical sensors to detect other biomolecules.