Single-cell assay on microfluidic devices. Issue 3 (4th September 2018)

• Record Type: Journal Article
• Authors: Huang, Qiushi; Mao, Sifeng; Khan, Mashooq; Lin, Jin-Ming
• Is Part Of: Analyst. Volume 144:Issue 3(2019)
• Page Start: 808
• Page End: 823
• Publication Date: 2018-09-04
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c8an01079j
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : Advances in microfluidic techniques have prompted researchers to study the inherent heterogeneity of single cells in cell populations. Abstract : Advances in microfluidic techniques have prompted researchers to study the inherent heterogeneity of single cells in cell populations. This would be helpful in the identification of major diseases and the design of personalized medicine. Different microfluidic approaches provide a variety of functions in the process of single-cell analysis. In this review, we take a broad overview of various microfluidic-based approaches for single-cell isolation, single-cell lysis, and single-cell analysis. Up-to-date flagship techniques and the pros and cons of these methods are discussed in detail.