Dalmanol biosyntheses require coupling of two separate polyketide gene clusters. Issue 1 (29th November 2018)

• Record Type: Journal Article
• Authors: Zhou, Zhen Zhen; Zhu, Hong Jie; Lin, Li Ping; Zhang, Xuan; Ge, Hui Ming; Jiao, Rui Hua; Tan, Ren Xiang
• Is Part Of: Chemical science. Volume 10:Issue 1(2019)
• Page Start: 73
• Page End: 82
• Publication Date: 2018-11-29
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c8sc03697g
• Languages: English
• ISSNs: 2041-6520

Abstract

Abstract : Polyketide–polyketide hybrids are unique natural products with promising bioactivity, but the hybridization processes remain poorly understood. Abstract : Polyketide–polyketide hybrids are unique natural products with promising bioactivity, but the hybridization processes remain poorly understood. Herein, we present that the biosynthetic pathways of two immunosuppressants, dalmanol A and acetodalmanol A, result from an unspecific monooxygenase triggered hybridization of two distinct polyketide (naphthalene and chromane) biosynthetic gene clusters. The orchestration of the functional dimorphism of the polyketide synthase (ChrA) ketoreductase (KR) domain (shortened as ChrA KR) with that of the KR partner (ChrB) in the bioassembly line increases the polyketide diversity and allows the fungal generation of plant chromanes ( e.g., noreugenin) and phloroglucinols ( e.g., 2, 4, 6-trihydroxyacetophenone). The simultaneous fungal biosynthesis of 1, 3, 6, 8- and 2-acetyl-1, 3, 6, 8-tetrahydroxynaphthalenes was addressed as well. Collectively, the work may symbolize a movement in understanding the multiple-gene-cluster involved natural product biosynthesis, and highlights the possible fungal generations of some chromane- and phloroglucinol-based phytochemicals.