Deep eutectic solvent as a green solvent for enhanced extraction of narirutin, naringin, hesperidin and neohesperidin from Aurantii Fructus. Issue 2 (13th November 2018)

• Record Type: Journal Article
• Authors: Liu, Yongjing; Zhang, Hua; Yu, Hongmin; Guo, Suhua; Chen, Dawei
• Is Part Of: Phytochemical analysis. Volume 30:Issue 2(2019)
• Page Start: 156
• Page End: 163
• Publication Date: 2018-11-13
• Subjects: Aurantii Fructus -- deep eutectic solvent -- hesperidin -- naringin -- narirutin -- neohesperidin; Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.2801
• Languages: English
• ISSNs: 0958-0344

Abstract

Abstract: Introduction: In the present study, a green and efficient extraction method using deep eutectic solvents as extraction solvent was developed for extracting the four major active compounds narirutin, naringin, hesperidin and neohesperidin from Aurantii Fructus. Methodology: A series of tunable deep eutectic solvents were prepared and investigated by mixing choline chloride or betaine to different hydrogen‐bond donors, and betaine/ethanediol was found to be the most suitable extraction solvent. To achieve the best extraction yield, the primary factors affecting the extraction efficiency, such as hydrogen‐bond acceptor/hydrogen‐bond donor ratio, water content in deep eutectic solvents, extraction temperature, solid/liquid ratio and extraction time, were investigated. Results: The optimal extraction conditions were 40% of water in betaine/ethanediol (1:4) at 60°C for heated extraction of 30 min and solid/liquid ratio 1:100 g/mL. Under the optimum extraction condition, the extraction yields of narirutin, naringin, hesperidin, and neohesperidin were 8.39 ± 0.61, 83.98 ± 1.92, 3.03 ± 0.35 and 35.94 ± 0.63 mg/g, respectively, which were much higher than those of methanol as extraction solvent (5.5 ± 0.48, 64.23 ± 1.51, 2.16 ± 0.15 and 30.14 ± 0.62 mg/g). Conclusion: The present results showed that deep eutectic solvents could be promising green and efficient solvents for extraction of the bioactive ingredients from traditional Chinese medicine. Abstract : A green and efficient extraction method using deep eutectic solvents as extraction solvent was developed for extracting the bioactive compounds from Aurantii Fructus.