Organelle-specific analysis of labile Fe(ii) during ferroptosis by using a cocktail of various colour organelle-targeted fluorescent probes. Issue 1 (14th September 2018)

• Record Type: Journal Article
• Authors: Hirayama, Tasuku; Miki, Ayaji; Nagasawa, Hideko
• Is Part Of: Metallomics. Volume 11:Issue 1(2019)
• Page Start: 111
• Page End: 117
• Publication Date: 2018-09-14
• Subjects: Metals -- Physiological effect -- Periodicals; 572.51
• DOI: 10.1039/c8mt00212f
• Languages: English
• ISSNs: 1756-5901

Abstract

Abstract : Fluorescence imaging analysis of labile Fe(ii ) during ferroptosis. Abstract : Ferroptosis is an emerging type of cell death mode that is dependent on iron. Unfortunately, the detailed analysis of the function of organelle labile Fe(ii ) in oxidative damage and lethality of the cells has not been demonstrated so far, mainly due to the lack of efficient methods to visualize labile Fe(ii ) at the targeted organelles. We have recently reported a series of Fe(ii )-selective fluorescent probes, i.e., Ac-MtFluNox, Lyso-RhoNox, and ER-SiRhoNox, which can detect Fe(ii ) specifically in the mitochondria, lysosomes, and endoplasmic reticulum (ER), respectively. These probes demonstrate similar reaction rates and off/on contrasts with various colours and intracellular distributions, enabling simultaneous multi-colour imaging that allows the monitoring of labile Fe(ii ) levels at each targeted organelle. In this paper, by using a cocktail of these probes, we successfully visualised the aberrant elevation of labile Fe(ii ) in the lysosomes and ER prior to HT1080 cell death induced by erastin, which is an inducer of ferroptosis.