A new visible-light-excitable ICT-CHEF-mediated fluorescence 'turn-on' probe for the selective detection of Cd2+ in a mixed aqueous system with live-cell imaging. Issue 12 (25th February 2015)

• Record Type: Journal Article
• Authors: Goswami, Shyamaprosad; Aich, Krishnendu; Das, Sangita; Das Mukhopadhyay, Chitrangada; Sarkar, Deblina; Mondal, Tapan Kumar
• Is Part Of: Dalton transactions. Volume 44:Issue 12(2015)
• Page Start: 5763
• Page End: 5770
• Publication Date: 2015-02-25
• Subjects: Chemistry, Inorganic -- Periodicals; Chemistry, Physical and theoretical -- Periodicals; Chemistry, Inorganic -- Periodicals; 546.05
• DOI: 10.1039/c4dt02463j
• Languages: English
• ISSNs: 1477-9226

Abstract

Abstract : A new quinoline based sensor was developed and applied for the selective detection of Cd 2+ both in vitro and in vivo . Abstract : A new quinoline based sensor was developed and applied for the selective detection of Cd 2+ both in vitro and in vivo . The designed probe displays a straightforward approach for the selective detection of Cd 2+ with a prominent fluorescence enhancement along with a large red shift (∼38 nm), which may be because of the CHEF (chelation-enhanced fluorescence) and ICT (internal charge transfer) processes after interaction with Cd 2+ . The interference from other biologically important competing metal ions, particularly Zn 2+, has not been observed. The visible-light excitability of the probe merits in the viewpoint of its biological application. The probe enables the detection of intracellular Cd 2+ with non-cytotoxic effects, which was demonstrated with the live RAW cells. The experimentally observed change in the structure and electronic properties of the sensor after the addition of Cd 2+ were modelled by the density functional theory (DFT) and time-dependent density functional theory (TDDFT) computational calculations, respectively. Moreover, the test strip experiment with this sensor exhibits both absorption and fluorescence color changes when exposed to Cd 2+ in a mixed aqueous solution, which also makes the probe more useful. The minimum limit of detection of Cd 2+ by the probe was in the range of 9.9 × 10 −8 M level.