Superb-selective chemodosimetric signaling of sulfide in the absence and in the presence of CT-DNA and imaging in living cells by a plant alkaloid berberine analogue. (18th January 2019)

• Record Type: Journal Article
• Authors: Jana, Gopal Chandra; Khatun, Munira; Nayim, Sk; Das, Somnath; Maji, Anukul; Beg, Maidul; Patra, Anirudha; Bhattacharjee, Paromita; Bhadra, Kakali; Hossain, Maidul
• Is Part Of: New journal of chemistry. Volume 43:Number 5(2019)
• Page Start: 2368
• Page End: 2380
• Publication Date: 2019-01-18
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c8nj06120c
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : New berberine analogue (BER-S), as a colorimetric probe in the absence of DNA and turn-on fluorometric probe in the presence of DNA towards S 2− detection is reported. Abstract : The present manuscript reports a lucrative design of a colorimetric and ratiometric chemodosimeter, 9- O -(2, 4-dinitrobenzenesulfonyl)berberrubine (BER-S), with excellent water solubility for the superb selective detection of sulfides through a color alteration from yellow to red with a good limit of detection (LOD) of 56 nM in CP buffer solution (10 mM, pH 7.2). Interestingly, this work also includes a smart "turn-on" emission probe (BER-S/DNA complex) showing good linearity with an excellent LOD of 46 nM for recognizing sulfide anions. The probes, BER-S, and BER-S/DNA complex, displayed no interfering effect by other analytes or sulfur-containing inorganic compounds, like thiols. Characterization was carried out using IR, HRMS, and DFT for the BER-S probe, and time-resolved fluorescence lifetime measurement and fluorescence titration for the BER-S/DNA complex probe for elucidating their sensing mechanism. The detection of S 2− in waste, tap, and drinking water by BER-S indicated its potential application in real sample analysis, while concentration variant cell imaging experiments (naked-eye red fluorescence) verified its cell-membrane permeability and capability for S 2− imaging in living cells. This reaction-based sensing strategy in the presence of DNA may provide a potential platform for the design of a fluorescent chemodosimeter for extensive anion targets.