Fast and sensitive fluorescent probe for ratiometric detection of hydrogen sulfide in mitochondria. Issue 2 (19th December 2018)

• Record Type: Journal Article
• Authors: Jiang, Na; Wang, Bin; Liu, Tao; Liu, Qingyu; Wei, Qin; Xing, Yujing; Zheng, Gengxiu
• Is Part Of: Analytical methods. Volume 11:Issue 2(2019)
• Page Start: 232
• Page End: 235
• Publication Date: 2018-12-19
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c8ay02586j
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : A hemicyanine-based fluorescent dye, Mito-BT, was developed to ratiometrically detect H2 S. This dye was of high selectivity, photostability and sensitivity with a rapid response time of 10 seconds. In addition, Mito-BT was localized in mitochondria, making it a promising probe for detecting H2 S in cells. Abstract : A probeMito-BT was constructed and synthesized by combining triphenylamine and hemicyanine, and can be used for specific ratiometric sensing of H2 S. It has a rapid response time of 10 s and preferentially targets to mitochondria. Then the visualization of the change of the mitochondrial H2 S concentration upon NaHS incubation by usingMito-BT staining is promising for the practical exploration of H2 S-related processes in mitochondria.