Fluorescence lifetime-activated droplet sorting in microfluidic chip systems. Issue 3 (3rd January 2019)

• Record Type: Journal Article
• Authors: Hasan, Sadat; Geissler, David; Wink, Konstantin; Hagen, Axel; Heiland, Josef J.; Belder, Detlev
• Is Part Of: Lab on a chip. Volume 19:Issue 3(2019)
• Page Start: 403
• Page End: 409
• Publication Date: 2019-01-03
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c8lc01278d
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : We present a highly efficient microfluidic fluorescence lifetime-activated droplet sorting (FLADS) approach as a novel technology for droplet manipulation in lab-on-a-chip devices. Abstract : We present a highly efficient microfluidic fluorescence lifetime-activated droplet sorting (FLADS) approach as a novel technology for droplet manipulation in lab-on-a-chip devices. In a proof-of-concept study, we successfully applied the approach to sort droplets containing two different fluorescent compounds on the basis of their corresponding fluorescence lifetime. Towards this end, a technical set-up was developed enabling on-the-fly fluorescence lifetime determination of passing droplets. The herein developed LabVIEW program enabled fast triggering of a downstream dielectrophoretic force sorting functionality depending on average fluorescence lifetimes of individual droplets. The approach worked reliably at individual substrate concentrations from 1 nM to 1 mM. This not only allowed reliable sorting of droplets containing species with different fluorescence lifetimes but also enabled differentiation of mixtures in individual droplets.