A continuous-flow acoustofluidic cytometer for single-cell mechanotyping. Issue 3 (16th January 2019)

• Record Type: Journal Article
• Authors: Wang, Han; Liu, Zhongzheng; Shin, Dong M.; Chen, Zhuo G.; Cho, Younghak; Kim, Yong-Joe; Han, Arum
• Is Part Of: Lab on a chip. Volume 19:Issue 3(2019)
• Page Start: 387
• Page End: 393
• Publication Date: 2019-01-16
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c8lc00711j
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : A continuous-flow single-cell mechanotyping method which can decouple the cell size-dependent effect from the cell compressibility-dependent effect is presented. Abstract : The biophysical properties of cells such as their compressibility have been found to be closely related to disease progression such as cancer development and metastasis. As cancer cells are heterogeneous, rapid and high-throughput evaluation of cell biophysical properties at single-cell resolution is needed to assess their potential as biomarkers for cancer staging and prognosis. Acoustofluidics has shown promise as a contactless method for accurately measuring cell biophysical properties; however, previously reported methods had relatively low throughput due to their requirement of no-flow conditions. This work presents a high-throughput continuous flow-based acoustofluidic cell mechanotyping method at single-cell resolution that retains the advantage of simplicity and low-cost.