Brucella canis induces canine CD4+ T cells multi-cytokine Th1/Th17 production via dendritic cell activation. (February 2019)

• Record Type: Journal Article
• Authors: Pujol, Myriam; Borie, Consuelo; Montoya, María; Ferreira, Arturo; Vernal, Rolando
• Is Part Of: Comparative immunology, microbiology and infectious diseases. Volume 62(2019)
• Page Start: 68
• Page End: 75
• Publication Date: 2019-02
• Subjects: Brucella canis -- CD4+ T cells -- Dendritic cells -- Host susceptibility -- Cytokines; Communicable diseases in animals -- Periodicals; Veterinary immunology -- Periodicals; Veterinary microbiology -- Periodicals; Immunology -- Periodicals; Microbiology -- Periodicals; Communicable diseases -- Periodicals; Communicable Diseases -- immunology -- Periodicals; Communicable Diseases -- veterinary -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Veterinary Medicine -- Periodicals; Immunologie -- Périodiques; Microbiologie -- Périodiques; Maladies infectieuses -- Périodiques; Communicable diseases; Immunology; Microbiology; Electronic journals; Periodicals; 636.08969
• DOI: 10.1016/j.cimid.2018.11.017
• Languages: English
• ISSNs: 0147-9571

Abstract

Graphical abstract: Highlights: Canine monocyte-derived DCs produce IL-12, IFN-γ, TNF-α and IL-17A against B. canis . Human B. canis -primed DCs induce Th1 cytokine production on autologous CD4 + T cells. Canine B. canis -primed DCs induce Th1 and Th17 cytokine production in autologous CD4 + T cells. Canine CD4 + T cells produce IFN-γ and IL-17A simultaneously after B. canis stimulation. Abstract: Brucella canis is a small intracellular Gram-negative bacterium that frequently leads to chronic infections highly resistant to antibiotic therapy in dogs. Also, it causes mild human brucellosis compared to other zoonotic Brucella spp. Herein we characterize the cellular immune response elicited by B. canis by analysing human and canine CD4 + T cells after stimulation with autologous monocyte-derived dendritic cells (MoDCs). Human and canine B. canis -primed MoDCs stimulated autologous CD4 + T cells; however, a Th1 response was triggered by human MoDCs, whereas canine MoDCs induced Th1/Th17 responses, with increased CD4 + T cells producing IFN-γ and IL-17A simultaneously. Each pattern of cellular response may contribute to host susceptibility, helping to understand the differences in B. canis virulence between these two hosts. In addition, other aspects of canine immunology are unveiled by highlighting the participation of IL-17A-producing canine MoDCs and CD4 + T cells producing IFN-γ and IL-17A.