(+)-Catechin potentiates the oxidative response of Acinetobacter baumannii to quinolone-based antibiotics. (February 2019)

• Record Type: Journal Article
• Authors: Ibitoye, O.B.; Ajiboye, T.O.
• Is Part Of: Microbial pathogenesis. Volume 127(2019)
• Page Start: 239
• Page End: 245
• Publication Date: 2019-02
• Subjects: Acinetobacter baumannii -- Superoxide dismutase -- Catalase -- (+)-catechin -- Glutathione -- Electron transport; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2018.12.012
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: (+)-Catechin is a versatile compound with its pharmacophore (catechol and resorcinol) responsible for prooxidant and antibacterial activities. In this study, we present that demonstrating the synergistic interaction between (+)-catechin and quinolone-based antibiotics, ciprofloxacin and gemifloxacin is related to reactive oxygen species generation. The minimum inhibitory concentration of (+)-catechin against Acinetobacter baumannii AB5075 were considerably lowered for Δ sodB and Δ katG mutants. Checkerboard assay shows synergistic interactions between (+)-catechin and quinolones. (+)-Catechin amplified quinolones-induced redox imbalance by increasing superoxide ion generation, NAD+/NADH ratio and ADP/ATP ratio. Conversely, the level of reduced glutathione was significantly lowered. We conclude that (+)-catechin potentiates quinolone-based antibiotics-induced oxidative stress in A. baumannii by increasing ROS generation, energy metabolism and electron transport chain activity with a concomitant decrease in glutathione. Graphical abstract: Image 1 Highlights: (+)- Catechin synergies quinolone-based antibiotics. (+)-Catechin enhanced quinolines-mediated reactive oxygen species generation. (+)- Catechin enhanced quinolines-induced respiratory chain activity.