Complete genome sequence of Burkholderia sp. JP2-270, a rhizosphere isolate of rice with antifungal activity against Rhizoctonia solani. (February 2019)

• Record Type: Journal Article
• Authors: Song, Dawei; Chen, Guoqing; Liu, Sihui; Khaskheli, Maqsood Ahmed; Wu, Lijuan
• Is Part Of: Microbial pathogenesis. Volume 127(2019)
• Page Start: 1
• Page End: 6
• Publication Date: 2019-02
• Subjects: Burkholderia -- Genome sequence -- Antagonistic activity -- lysR-type transcriptional regulator -- Rice sheath blight; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2018.11.024
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Burkholderia sp. JP2-270, a bacterium with a strong ability to inhibit the growth of Rhizoctonia solani, was isolated from the rhizosphere of rice. The phylogenetic analysis based on 16S rRNA gene revealed that JP2-270 belonged to Burkholderia cepacia complex. Here, we present the complete genome sequence of Burkholderia sp. JP2-270, which consists of three circular chromosomes (Chr1 3, 723, 585 bp, Chr2 3, 274, 969 bp, Chr3 1, 483, 367 bp) and two plasmids (Plas1 15, 126 bp, Plas2 428, 263 bp). A total of 8193 protein coding genes were predicted in the genome, including 67 tRNA genes, 18 rRNA genes and 4 ncRNA genes. In addition, mutation analysis of Burkholderia sp. JP2-270 revealed that the gene bysR (DM992_17470), encoding a lysR-type transcriptional regulator, was essential for the antagonistic activity of Burkholderia sp. JP2-270 against R. solani GD118 in vitro and in vivo . Identification of regulatory gene associated with antagonistic activity will contribute to understand the antagonistic mechanism of Burkholderia sp. JP2-270. Highlights: We report the complete genome sequence of a new isolate of Burkholderia cepacia complex JP2-270. The Burkholderia sp. JP2-270 genome consists of three circular chromosomes (Chr1 3, 723, 585 bp, Chr2 3, 274, 969 bp, Chr3 1, 483, 367 bp) and two plasmids (Plas1 15, 126 bp, Plas2 428, 263 bp). Identification of bysR as an important player in the antagonistic activity of Burkholderia sp. JP2-270.