Inactivation of biofilm-bound Pseudomonas aeruginosa bacteria using UVC light emitting diodes (UVC LEDs). (15th March 2019)

• Record Type: Journal Article
• Authors: Gora, Stephanie L.; Rauch, Kyle D.; Ontiveros, C. Carolina; Stoddart, Amina K.; Gagnon, Graham A.
• Is Part Of: Water research. Volume 151(2019)
• Page Start: 193
• Page End: 202
• Publication Date: 2019-03-15
• Subjects: Disinfection -- Biofilm -- UV -- Light emitting diodes -- UV LED; Water -- Pollution -- Research -- Periodicals; 363.7394
• DOI: 10.1016/j.watres.2018.12.021
• Languages: English
• ISSNs: 0043-1354

Abstract

Abstract: Ultraviolet light emitting diodes (UV LEDs) are a promising technology for the disinfection of water and wetted surfaces, but research into these applications remains limited. In the drinking water field, UV LEDs emitting at wavelengths ranging from 254 nm to 285 nm (UVC LEDs) have been shown to be effective for the inactivation of numerous pathogens and pathogen surrogate organisms at UV doses comparable to conventional germicidal UV lamps. Surface disinfection with UV light, from UVC LEDs or from conventional UV lamps, is not as well understood. As the technology underlying the design and construction of UV LEDs matures and their energy efficiency improves, it is likely that they will become ubiquitous in small scale water treatment applications and surface disinfection in various industries, including the medical and dental fields. A simple, easily replicated methodology was developed and optimized to grow, irradiate, and recover biofilms from coupons. It was hypothesized that higher UV doses would be required to inactivate biofilm-bound bacteria than planktonic (free-floating) bacteria because the biofilm would provide some degree of protection from the effects of UVC irradiation. Indeed, UV LED irradiation at 265 nm achieved 1.3 ± 0.2 log inactivation of biofilm-bound Pseudomonas aeruginosa at a UV dose of 8 mJ/cm 2 . This inactivation level is lower than those that have been reported by researchers using UVC LEDs to inactivate planktonic P. aeruginosa, a finding that can be explained by the higher resistance of biofilm-bound bacteria to UV inactivation. A dose-response curve was developed and fitted to three disinfection models: the Chick-Watson model, the multi-target model, and the Geeraerd model. This last, which posits a subpopulation of organisms that are resistant to treatment, was a good fit to the dose-response data. ATP results obtained using the biomass recovery ATP method (ATPBR ), a method that includes a 4 h incubation period after treatment, was well correlated to the results of conventional plate counts. Graphical abstract: Image 1 Highlights: A methodology for UVC LED inactivation of biofilm-bound bacteria was developed. UVC LED irradiation at 265 nm inactivated biofilm-bound Pseudomonas aeruginosa . The dose-response curve for P. aeruginosa was a good fit to the Geeraerd model. Plate count results were correlated to ATP measured after 4 h of incubation.