Control of bacterial attachment by fracture topography. (March 2019)

• Record Type: Journal Article
• Authors: Velic, Amar; Mathew, Asha; Hines, Peter; Yarlagadda, Prasad K.D.V.
• Is Part Of: Journal of the mechanical behavior of biomedical materials. Volume 91(2019)
• Page Start: 416
• Page End: 424
• Publication Date: 2019-03
• Subjects: Titanium alloy -- Fractography -- Bacterial adhesion -- Staphylococcus aureus -- Antibiofouling -- Topography; Biomedical materials -- Periodicals; Biomedical materials -- Mechanical properties -- Periodicals; Biomedical materials; Biomedical materials -- Mechanical properties; Periodicals; Electronic journals; 610.28
• DOI: 10.1016/j.jmbbm.2018.10.020
• Languages: English
• ISSNs: 1751-6161

Abstract

Abstract: In the biomedical arena, bacterial fouling is a precursor to complications such as implant infection and nosocomial infection. These complications are further compounded by biochemical mechanisms of resistance that threaten the action of traditional antibacterial strategies. Accordingly, antibacterial property by physical, not biochemical, mechanisms of action is becoming increasingly popular and promising. The present work falls in line with this paradigm shift. Here, microtextured Ti-6Al-4V surfaces were manufactured by destructive tension at three different cross-head speeds, probed with scanning electron microscopy (SEM) and multifocus optical microscopy, and treated with Staphylococcus aureus to study bacterial attachment. The fractographic study revealed the presence of dual-mode fracture, typical of Ti-6Al-4V,  comprising  regions of both ductile, microvoid coalescence and brittle, cleavage faceting. Based on load-extension curves, quantitative roughness data, and qualitative SEM visualisation, it was evident that cross-head speed modulated fracture behaviour such that increased speed produced more brittle fracture whilst lower speeds produced more ductile fracture. The topography associated with ductile fracture was found to possess notable antibiofouling property due to geometric constrains imposed by the coalesced microvoids. Accordingly, fracture at low cross-head speeds (1 mm/min and 10 mm/min) yielded significant reduction in bacterial attachment, whilst fracture at high cross-head speeds (100 mm/min) did not. The greatest reduction (~72%) was achieved at a cross-head speed of 1 mm/min. These findings suggest that antibiofouling property can be elicited by fracture and further 'tuned' by fracture speed. Discovery of this novel, albeit simple, avenue for topography-mediated antibacterial property calls for further research into alternate techniques for the manufacture of 'physical antibacterial surfaces'. Graphical abstract: fx1 Highlights: Textured Ti-6Al-4V surfaces were made by tensile fracture at three cross-head speeds. Higher cross-head speeds elicited more brittle, cleavage faceting. Lower cross-head speeds elicited more ductile, microvoid coalescence. Coalesced microvoids exhibited topography-mediated antibiofouling property. Significant reduction in attached bacteria was achieved at lower cross-head speeds.