Fluence rate dependence of red light-induced phosphorylation of plasma membrane H+-ATPase in stomatal guard cells. (1st February 2019)

• Record Type: Journal Article
• Authors: Ando, Eigo; Kinoshita, Toshinori
• Is Part Of: Plant signaling & behavior. Volume 14:Number 2(2019)
• Publication Date: 2019-02-01
• Subjects: Arabidopsis thaliana -- red light -- photosynthesis -- guard cells -- immunohistochemistry -- phosphorylation -- plasma membrane H+-ATPase; Plant ecophysiology -- Periodicals; Plant cellular signal transduction -- Periodicals; Plant cellular signal transduction; Plant ecophysiology; Periodicals; Electronic journals; 581
• DOI: 10.1080/15592324.2018.1561107
• Languages: English
• ISSNs: 1559-2316

Abstract

ABSTRACT: Stomatal opening is induced by red light as well as blue light. Recently, we established an immunohistochemical technique using whole leaves to study plasma membrane (PM) H + -ATPase in guard cells, which is an important enzyme driving stomatal opening. Our technique revealed that red light illuminated to whole leaves induces photosynthesis-dependent phosphorylation of C-terminal penultimate residue of PM H + -ATPase, threonine, in guard cells, which has been considered to be important for activation of PM H + -ATPase, and we proposed that red light promotes stomatal opening via activation of PM H + -ATPase in guard cells in whole leaves. Here, using our new immunohistochemical technique, we investigated fluence rate dependence of red light-induced phosphorylation of PM H + -ATPase. We found that illumination of red light at 50 µmol m –2 s –1, which was suggested to initiate photosynthesis, saturates phosphorylation of PM H + -ATPase. Furthermore, we immunohistochemically confirmed decrease in the amount of PM H + -ATPase protein in a knock-out mutant of AHA1, an isogene encoding the major isoform of PM H + -ATPase in guard cells, implying the importance of AHA1 as the major PM H + -ATPase protein in guard cells for light-induced stomatal opening.