Satureja montana L. essential oil and its antimicrobial activity alone or in combination with gentamicin. (January 2019)

• Record Type: Journal Article
• Authors: Vitanza, Luca; Maccelli, Alessandro; Marazzato, Massimiliano; Scazzocchio, Francesca; Comanducci, Antonella; Fornarini, Simonetta; Crestoni, Maria Elisa; Filippi, Antonello; Fraschetti, Caterina; Rinaldi, Federica; Aleandri, Marta; Goldoni, Paola; Conte, Maria Pia; Ammendolia, Maria Grazia; Longhi, Catia
• Is Part Of: Microbial pathogenesis. Volume 126(2019)
• Page Start: 323
• Page End: 331
• Publication Date: 2019-01
• Subjects: Metabolomics -- FTICR-MS -- Synergism -- Escherichia coli -- Listeria monocytogenes -- Staphylococcus aureus; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2018.11.025
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Many essential oils (EOs) are screened as potential sources of antimicrobial compounds. EOs from the genus Satureja have recognized biological properties, including analgesic, anti-inflammatory, immunomodulatory, anticancer, and antimicrobial activity. This study aimed to obtain a metabolite profile of commercial essential oil of S. montana L. (SEO) and to evaluate its antimicrobial properties, both alone and combined with gentamicin towards Gram-negative and Gram-positive bacterial strains. Untargeted analyses based on direct infusion Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS) and on GC-MS have provided a high metabolome coverage, allowing to identify carvacrol, cymene and thymol as the major components of commercial SEO. SEO exerted an antimicrobial activity and induced a synergistic interaction with gentamicin against both reference and clinical bacterial strains. A significant reduction of Escherichia coli, Staphylococcus aureus and Listeria monocytogenes biofilm formation was induced by SEO. As a result of SEO treatment, clear morphological bacterial alterations were visualized by scanning electron microscopy: L. monocytogenes and S. aureus showed malformed cell surface or broken cells with pores formation, whereas E. coli displayed collapsed cell surface. These results encourage further studies about bactericidal and antibiotic synergistic effect of SEO for combined therapy in clinical setting as well as in agricultural systems. Graphical abstract: Highlights: Carvacrol, cymene and thymol are major components of Satureja montana essential oil. S. montana essential oil shows antimicrobial activity. S. montana essential oil exerts synergistic interaction with gentamicin against bacterial strains. Reduction of bacterial biofilm formation was induced by S. montana essential oil.