Using open‐tubular capillary electrochromatography with part‐coating column for binding constants determination of β2‐adrenergic receptor with seven drugs. Issue 2 (23rd October 2018)

• Record Type: Journal Article
• Authors: Liu, Chunye; Zhang, Xuejiao; Jing, Hui; Zhang, Jingshui; Miao, Yanqing; Zhai, Xifeng; Chen, Songyi
• Is Part Of: Electrophoresis. Volume 40:Issue 2(2019)
• Page Start: 289
• Page End: 295
• Publication Date: 2018-10-23
• Subjects: Affinity interaction -- Drug activity -- Open‐tubular capillary electrochromatography -- Oriented covalent immobilization; Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201800207
• Languages: English
• ISSNs: 0173-0835

Abstract

Abstract: An open‐tubular capillary electrochromatography method has been developed for the determination of binding constants between β 2 ‐adrenergic receptor ( β 2 ‐AR) and seven drugs . β 2 ‐AR was oriented immobilized onto one part of inner surface of capillary via microwave‐assisted technical synthesis. According to the linear relationship between coating length and the apparent mobility of analyte, the binding constant ( K b ) can be obtained by related theories and equations. The order of K b values between drugs such as adrenaline hydrochloride, norepinephrine bitartrate, and propranolol hydrochloride with β 2 ‐AR is well consistent with that reported in the literature. By the method, K b values between four extracts of Radix Paeoniae Rubra and β2 ‐ AR were also successfully obtained. Subsequently, computer models were applied to interpret the CEC experiments. And the results proved to be in good agreement with the method. The work, herein, demonstrates the potential of the method in drug‐receptor affinity interactions evaluation and screening of lead compounds from natural sources.