Characterization of Brain Metabolism by Nuclear Magnetic Resonance. Issue 2 (20th December 2018)

• Record Type: Journal Article
• Authors: Downes, Daniel P.; Collins, James H. P.; Lama, Bimala; Zeng, Huadong; Nguyen, Tan; Keller, Gabrielle; Febo, Marcelo; Long, Joanna R.
• Is Part Of: Chemphyschem. Volume 20:Issue 2(2019)
• Page Start: 216
• Page End: 230
• Publication Date: 2018-12-20
• Subjects: brain metabolism -- dynamic nuclear polarization -- in vivo and ex vivo metabolomics -- magnetic resonance spectroscopy -- neurochemistry; Chemistry, Physical and theoretical -- Periodicals; 541.05
• DOI: 10.1002/cphc.201800917
• Languages: English
• ISSNs: 1439-4235

Abstract

Abstract: The noninvasive, quantitative ability of nuclear magnetic resonance (NMR) spectroscopy to characterize small molecule metabolites has long been recognized as a major strength of its application in biology. Numerous techniques exist for characterizing metabolism in living, excised, or extracted tissue, with a particular focus on 1 H‐based methods due to the high sensitivity and natural abundance of protons. With the increasing use of high magnetic fields, the utility of in vivo 1 H magnetic resonance spectroscopy (MRS) has markedly improved for measuring specific metabolite concentrations in biological tissues. Higher fields, coupled with recent developments in hyperpolarization, also enable techniques for complimenting 1 H measurements with spectroscopy of other nuclei, such as 31 P and 13 C, and for combining measurements of metabolite pools with metabolic flux measurements. We compare ex vivo and in vivo methods for studying metabolism in the brain using NMR and highlight insights gained through using higher magnetic fields, the advent of dissolution dynamic nuclear polarization, and combining in vivo MRS and ex vivo NMR approaches. Abstract : Brain metabolism : This minireview compare ex vivo and in vivo methods for studying metabolism in the brain using NMR, We highlight insights gained through using higher magnetic fields, the advent of dissolution dynamic nuclear polarization, and combining in vivo MRS and ex vivo NMR approaches.