A study of viral coat protein accumulation in lily chloroplasts from mixed virus infections of Lily mottle virus and Cucumber mosaic virus. (26th October 2018)

• Record Type: Journal Article
• Authors: Zhang, Y. B.; Wang, Y. J.; Xie, Z. K.; Wang, R. Y.; Yang, G.; Guo, Z. H.; Qiu, Y.
• Is Part Of: Plant pathology. Volume 68:Number 2(2019)
• Page Start: 261
• Page End: 268
• Publication Date: 2018-10-26
• Subjects: chloroplast -- coat protein -- Cucumber mosaic virus -- Lilium spp. -- Lily mottle virus -- mixed virus infections; Agricultural pests -- Periodicals; Plant diseases -- Periodicals; 632
• DOI: 10.1111/ppa.12940
• Languages: English
• ISSNs: 0032-0862

Abstract

Abstract : Two viruses that frequently occur in many Lilium species are Lily mottle virus (LMoV) and Cucumber mosaic virus (CMV), which usually co‐infect lilies causing severe disease symptoms. Recent reports have revealed that the viral coat protein (CP) affects chloroplast ultrastructure and symptom development. This study used western blot analysis to confirm that in leaves infected by mixed virus infections of LMoV and CMV, CPs of both viruses were accumulated in lily chloroplasts. Immunogold labelling further demonstrated that both the LMoV CP and CMV CP were localized in the stroma and the thylakoid membranes of the chloroplasts. In addition, it was found that CPs of both viruses were rapidly transported into isolated, intact chloroplasts ( in vitro ), and their transport efficiencies were positively related to CP concentrations. The lowest transmembrane concentration of CMV CP decreased from 38 μg mL −1 recorded in the single CMV CP import system to 10 μg mL −1 in the mixed import system of LMoV CP and CMV CP. CPs of both viruses exhibited species selection in their transmembrane transport into chloroplasts. This is the first report that the CPs from two viruses (LMoV and CMV) are simultaneously present in lily chloroplasts. Accumulation of high levels of LMoV CP and CMV CP inside the chloroplast appears to contribute to a synergistic interaction inducing the development of mosaic symptoms.