Magnesium isoglycyrrhizinate suppresses LPS-induced inflammation and oxidative stress through inhibiting NF-κB and MAPK pathways in RAW264.7 cells. Issue 3 (1st February 2019)

• Record Type: Journal Article
• Authors: Xie, Chunfeng; Li, Xiaoting; Zhu, Jianyun; Wu, Jieshu; Geng, Shanshan; Zhong, Caiyun
• Is Part Of: Bioorganic & medicinal chemistry. Volume 27:Issue 3(2019)
• Page Start: 516
• Page End: 524
• Publication Date: 2019-02-01
• Subjects: MgIG Magnesium isoglycyrrhizinate -- LPS lipopolysaccharide -- NF-κB Nuclear factor-κB -- MAPK mitogen-activated protein kinases -- ROS reactive oxygen species -- TNF-α tumor necrosis factor-α -- iNOS nitric oxide synthase -- ERK1/2 extracellular signal-regulated kinase 1/2 -- JNK c-Jun NH2-terminal kinase; Magnesium isoglycyrrhizinate -- Inflammation -- Reactive oxygen species -- NF-κB -- MAPK; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; Biochemistry -- Periodicals; Chemistry, Clinical -- Periodicals; Chemistry, Organic -- Periodicals; Chimie bio-organique -- Périodiques; Chimie pharmaceutique -- Périodiques; 615.19
• DOI: 10.1016/j.bmc.2018.12.033
• Languages: English
• ISSNs: 0968-0896

Abstract

Graphical abstract: Abstract: Magnesium Isoglycyrrhizinate (MgIG), a novel molecular compound extracted from licorice root, has exhibited greater anti-inflammatory activity and hepatic protection than glycyrrhizin and β-glycyrrhizic acid. In this study, we investigated the anti-inflammatory effect and the potential mechanism of MgIG on Lipopolysaccharide (LPS)-treated RAW264.7 cells. MgIG down-regulated LPS-induced pro-inflammatory mediators and enzymes in LPS-treated RAW264.7 cells, including TNF-α, IL-6, IL-1β, IL-8, NO and iNOS. The generation of reactive oxygen species (ROS) in LPS-treated RAW264.7 cells was also reduced. MgIG attenuated NF-κB translocation by inhibiting IKK phosphorylation and IκB-α degradation. Simultaneously, MgIG also inhibited LPS-induced activation of MAPKs, including p38, JNK and ERK1/2. Taken together, these results suggest that MgIG suppresses inflammation by blocking NF-κB and MAPK signaling pathways, and down-regulates ROS generation and inflammatory mediators.