Bioassay-guided isolation of anti-seizure principles from Semen Pharbitidis using a zebrafish pentylenetetrazol seizure model. (25th March 2019)

• Record Type: Journal Article
• Authors: Liu, Maoxuan; Copmans, Daniëlle; Lu, Jing-Guang; Yang, Ming-Rong; Sourbron, Jo; Ny, Annelii; Jiang, Zhi-Hong; de Witte, Peter A.M.; Luyten, Walter
• Is Part Of: Journal of ethnopharmacology. Volume 232(2019)
• Page Start: 130
• Page End: 134
• Publication Date: 2019-03-25
• Subjects: Anti-seizure -- Zebrafish -- Semen Pharbitidis -- Pharbitin; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2018.12.024
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Semen Pharbitidis, the seeds of Pharbitis nil (Linn.) Choisy (Convolvulaceae) is a well-known traditional Chinese medicinal plant used for treating helminthiasis and epilepsy in China. Aim of the study: This study aims to identify the anti-seizure components from Semen Pharbitidis . Methods: A bioassay-guided isolation of anti-seizure compounds from Semen Pharbitidis was performed using a zebrafish pentylenetetrazol seizure model. The structures of active compounds were elucidated by high resolution mass spectrometry. The fragments of active compounds were tested for anti-seizure activity as well. Results: The bioassay-guided isolation of ethanol extract of Semen Pharbitidis led to a group of resin glucosides, namely pharbitin. One of the fragments of pharbitin, 2-methylbutyric acid, also showed anti-seizure activity. Conclusions: We provided further experimental scientific evidence to support the traditional use of Semen Pharbitidis for the treatment of epilepsy. Pharbitin was identified to be the main anti-seizure component in Semen Pharbitidis . Graphical abstract: