Using CRISPR/Cas9 genome editing in tomato to create a gibberellin‐responsive dominant dwarf DELLA allele. Issue 1 (22nd June 2018)

• Record Type: Journal Article
• Authors: Tomlinson, Laurence; Yang, Ying; Emenecker, Ryan; Smoker, Matthew; Taylor, Jodie; Perkins, Sara; Smith, Justine; MacLean, Dan; Olszewski, Neil E.; Jones, Jonathan D. G.
• Is Part Of: Plant biotechnology journal. Volume 17:Issue 1(2019)
• Page Start: 132
• Page End: 140
• Publication Date: 2018-06-22
• Subjects: CRISPR/Cas9 -- DELLA -- gibberellic acid -- dwarf -- slender -- PROCERA; Plant biotechnology -- Periodicals; Plant genetic engineering -- Periodicals; 630.272
• DOI: 10.1111/pbi.12952
• Languages: English
• ISSNs: 1467-7644

Abstract

Summary: The tomato PROCERA gene encodes a DELLA protein, and loss‐of‐function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss‐of‐function mutations and a dominant dwarf mutation that carries a deletion of one amino acid in the DELLA domain. This is the first report of a dominant dwarf PROCERA allele. This allele retains partial responsiveness to exogenously applied gibberellin. Heterozygotes show an intermediate phenotype at the seedling stage, but adult heterozygotes are as dwarfed as homozygotes.