Foliar epidermal micromorphology and its taxonomic implications in some selected species of Athyriaceae. Issue 8 (10th July 2018)

• Record Type: Journal Article
• Authors: Shah, Syed Nasar; Ahmad, Mushtaq; Zafar, Muhammad; Razzaq, Abdul; Malik, Khafsa; Rashid, Neelam; Ullah, Fazal; Iqbal, Majid; Zaman, Wajid
• Other Names: Perry George specialEditor.
• Is Part Of: Microscopy research and technique. Volume 81:Issue 8(2018)
• Page Start: 902
• Page End: 913
• Publication Date: 2018-07-10
• Subjects: Athyriaceae -- foliar epidermis -- LM -- Pakistan -- SEM -- taxonomic keys; Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.23055
• Languages: English
• ISSNs: 1059-910X

Abstract

Abstract: For the robust identification of taxonomically complex fern family like Athyriaceae, light and scanning electron microscopy is significance implications. This article present first microscopic investigation of foliar micromorphology of 3 genera and 10 species belonging to Athyriaceae namely, Athyrium, Deparia, and Diplazium were collected from different localities in Malakand Division, Northern Pakistan. In present study we compare foliar micromorphology of all 10 species using standard protocols of light microcopy (LM) and scanning electron microscopy. Qualitative micromorphological variations in shape of epidermal cells, anticlinal wall pattern, stomatal type and shape, stomatal pore shape, guard cells shape, and trichomes types were studied. In addition, some quantitative characters were also studied and data were statistically analyzed in epidermal cell size, stomatal size, stomatal pore size, stomatal density, and stomatal index. The pivotal result of study include; shape of epidermal cell in all species is irregular on both abaxial and adaxial surfaces. The anticlinal walls are sinuous in most of the species but some species have irregular lobed and broadly lobed wall. Leaves are hypostomatic in all studied species. Two main categories of stomatal type were found: polocytic and anomocytic. Unicellular nonglandular trichomes were observed in only one species Athyrium mackinnoni . The variation in foliar micromorphological characters between the genera and within the species was useful in identification and classification and have potential taxonomic significance for species differentiation. An identification key using micromorphological characters are provided to distinguish genera and species. Abstract : First microscopic investigation and their taxonomic significance in some selected species of Pakistani fern family Athyriaceae Micromorphological characters by using scanning electron microscopy (SEM) and light microcopy (LM) for taxonomic purposes in fern family Athyriaceae Taxonomic segregation of genera and species and their identification key based of leaf micromorphological characters