Molecular characterization of virulence and antibiotic resistance genes among Salmonella serovars isolated from broilers in Egypt. (6th January 2019)

• Record Type: Journal Article
• Authors: Ammar, A.M.; Abdeen, E.E.; Abo‐Shama, U.H.; Fekry, E.; Kotb Elmahallawy, E.
• Is Part Of: Letters in applied microbiology. Volume 68:Number 2(2019)
• Page Start: 188
• Page End: 195
• Publication Date: 2019-01-06
• Subjects: broilers -- genes -- PCR -- resistance -- Salmonella -- serotyping -- virulence; Microbiology -- Periodicals; 660.62
• DOI: 10.1111/lam.13106
• Languages: English
• ISSNs: 0266-8254

Abstract

Abstract: This study was carried out to investigate the resistance to some antimicrobial groups among Salmonellae isolated from broilers in Egypt. The prevalence of some virulence and resistance genes among the recovered multidrug resistant (MDR) isolates was also scrutinized. A total of 55 (15·6%) Salmonella isolates were recovered from 353 different samples (liver, yolk sac, gall bladder and caecum), gathered from apparently healthy and diseased broilers suffered from diarrhoea, dehydration and respiratory distress. Thirty Salmonellae strains were serotyped into Salmonella E nteritidis, Salmonella Infantis, Salmonella Kentucky, Salmonella Maloma, Salmonella Bardo, Salmonella Gdansk, Salmonella Typhimurium and Salmonella Blegdame. The resistance pattern of all Salmonella isolates was constructed and 15 MDR Salmonella isolates were subsequently examined for the presence of virulence ( inv A, omp A and stn ) and resistance ( qnr S, qnr A, bla TEM and bla CTX ) genes. Of note, inv A, omp A and stn virulence genes and bla TEM and qnr S resistance genes were found in all examined isolates. On the other hand, the qnr A gene detection frequency was 20%, whereas bla CTX was not detected at all. Our findings emphasize the wide spread of antimicrobial resistance genes in Salmonella isolates and the importance of effective control measures for the disease. Significance and Impact of the Study: This study highlights the possible emergence of widespread resistance to some antimicrobials among Salmonellae isolated from broilers in Egypt. The results also reveal the prevalence of some virulence ( inv A, omp A and stn ) and resistance ( qnr S, qnr A, bla CTX and bla TEM ) genes among the recovered multidrug resistant isolates. Clearly, our data emphasize that antimicrobial resistance genes are widely spread in Salmonella isolates which is crucial for developing novel methods for controlling such disease problem of zoonotic concern. Abstract : Significance and Impact of the Study: This study highlights the possible emergence of widespread resistance to some antimicrobials among Salmonellae isolated from broilers in Egypt. The results also reveal the prevalence of some virulence ( inv A, omp A and stn ) and resistance ( qnr S, qnr A, bla CTX and bla TEM ) genes among the recovered multidrug resistant isolates. Clearly, our data emphasize that antimicrobial resistance genes are widely spread in Salmonella isolates which is crucial for developing novel methods for controlling such disease problem of zoonotic concern.