Intracellular uranium distribution: Comparison of cryogenic fixation versus chemical fixation methods for SIMS analysis. Issue 8 (8th May 2018)

• Record Type: Journal Article
• Authors: Suhard, D.; Tessier, C.; Manens, L.; Rebière, F.; Tack, K.; Agarande, M.; Guéguen, Y.
• Other Names: Verkade Paul specialEditor.
• Is Part Of: Microscopy research and technique. Volume 81:Issue 8(2018)
• Page Start: 855
• Page End: 864
• Publication Date: 2018-05-08
• Subjects: cell culture -- ion imaging -- sample preparations -- uranium; Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.23047
• Languages: English
• ISSNs: 1059-910X

Abstract

Abstract: Localization of uranium within cells is mandatory for the comprehension of its cellular mechanism of toxicity. Secondary Ion Mass Spectrometry (SIMS) has recently shown its interest to detect and localize uranium at very low levels within the cells. This technique requires a specific sample preparation similar to the one used for Transmission Electronic Microscopy, achieved by implementing different chemical treatments to preserve as much as possible the living configuration uranium distribution into the observed sample. This study aims to compare the bioaccumulation sites of uranium within liver or kidney cells after chemical fixation and cryomethods preparations of the samples: SIMS analysis of theses samples show the localization of uranium soluble forms in the cell cytoplasm and nucleus with a more homogenous distribution when using cryopreparation probably due to the diffusible portion of uranium inside the cytoplasm. Abstract : The use of the cryogenic and chemical fixation is compared to analyze the micro‐distribution of uranium at low level by Secondary Ion Mass Spectrometry. Intra‐nuclear localization of soluble uranium is observed using both technics of fixation with a more homogenous intracellular distribution when cells were cryofixed.