Effects of tumor necrosis factor‐α‐induced exosomes on the endothelial cellular behavior, metabolism and bioenergetics. (29th November 2018)

• Record Type: Journal Article
• Authors: Li, Bingwei; Li, Luhan; Zhang, Qiuju; Zhang, Honggang; Xiu, Ruijuan
• Is Part Of: Microcirculation. Volume 26:Number 1(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-11-29
• Subjects: bioenergetics -- cell behavior -- endothelial cells -- exosomes -- TNF‐α; Biological transport -- Periodicals; Microcirculation -- Physiology -- Periodicals; 612.135
• DOI: 10.1111/micc.12515
• Languages: English
• ISSNs: 1073-9688

Abstract

Abstract: Objective: To investigate the effects of TNF‐α‐induced exosomes release on the biological behavior, metabolism, and bioenergetics of HUVECs. Methods: Exosomes were isolated from conditioned media of HUVECs by ultracentrifugation after treatment with or without TNF‐α. HUVECs were treated with or without TNF‐α, or different concentrations of exosomes isolated from conditioned media with or without TNF‐α induction (TExo and CExo, respectively). Results: The results showed that TNF‐α significantly inhibited migration, tube formation, and increased apoptosis rate of HUVECs compared with controls. Furthermore, TNF‐α‐induced exosomes (TExo ) rather than CExo, indicated similar effects to inhibit migration, tube formation and promote endothelial apoptosis. Although TNF‐α treatment did not show a statistical difference, TExo significantly inhibited extracellular OCR compared with controls. TExo could significantly inhibited intracellular OCR in a hypoxia condition. TNF‐α significantly increased L‐ECA compared with control cells, and TExo showed similar stimulative effect on L‐ECA. Conclusions: TNF‐α‐induced exosomes could significantly (a) change migration, tube formation, and apoptosis; (b) inhibit endothelial extracellular OCR and intracellular OCR (hypoxia); (c) increase glycolysis rate of the endothelial cells. These data provide new evidence for exploring endothelial behavior regulation using exosomes and their effects on endothelial metabolism and bioenergetics.