Autologous canine immunotherapy: short-time generated dendritic cells loaded with canine transmissible venereal tumor-whole lysate. (3rd September 2018)

• Record Type: Journal Article
• Authors: Franco-Molina, Moisés Armides; Ramos-Zayas, Yareellys; Coronado-Cerda, Erika Evangelina; Mendoza-Gamboa, Edgar; Zapata-Benavides, Pablo; Santana-Krymskaya, Silvia Elena; Tamez-Guerra, Reyes; Rodríguez-Padilla, Cristina
• Is Part Of: Immunopharmacology and immunotoxicology. Volume 40:Number 5(2018)
• Page Start: 437
• Page End: 443
• Publication Date: 2018-09-03
• Subjects: Immunotherapy -- dendritic cells -- canine tumor venereal transmissible -- interferon-γ -- lymphocytes; Immunopharmacology -- Periodicals; Immunotoxicology -- Periodicals; Antibody-toxin conjugates -- Periodicals; Immunology -- Periodicals; 615.37
• DOI: 10.1080/08923973.2018.1523928
• Languages: English
• ISSNs: 0892-3973

Abstract

Abstract: Objective: The aim of the present study was to evaluate the therapeutic potential of autologous DCs loaded with whole tumor cell lysate of CTVT generated under a simplified and rapid procedure in vitro production process, in a vulvar submucosal model of CTVT in dogs. Materials and methods: We generated a model of intravulvar CTVT in dogs. A CTVT lysate antigen was prepared according to the method of 1-butanol and after administered with complete Freund's adjuvant via subcutaneous in female healthy dogs and challenge with CTVT cells to corroborate the immunogenicity. Short-time generated dendritic cell pulsed with CTVT whole-lysate was performed, and analyzed by FITC-dextran uptake assay and characterized using anti-canine monoclonal antibodies CD14, CD80, CD83, and DLAII by flow cytometry. Dendritic cell therapy was administered in a frequency of three times every 2 weeks when the CTVT had 4 months of growth and 89 ± 5 cm diameter. The CD3 +, CD4 + and CD8 + lymphocytes were determined by flow cytometry, and IFN-γ by ELISA assay. Results and discussion: The administration of CTVT whole-lysate resulted in tumor prevention. The short-time generated dendritic cell pulsed with CTVT whole-lysate administration resulted in an efficient reduction and elimination of CTVT, probably due to the increase in lymphocyte populations (CD3 +, CD4 +, and CD8 + ), IFN-γ production and tumor infiltrating lymphocytes. Conclusion: In conclusion, this study demonstrates the efficacy of immunotherapy based in short-time generated dendritic cell pulsed with CTVT whole-lysate for the treatment of CTVT, and offer veterinary oncologists new alternative therapies to treat this and another malignancy.