Concanavalin-A displays leishmanicidal activity by inducing ROS production in human peripheral blood mononuclear cells. (3rd September 2018)

• Record Type: Journal Article
• Authors: Thomazelli, Ana Paula Fortes dos Santos; Tomiotto-Pellissier, Fernanda; Miranda-Sapla, Milena Menegazzo; da Silva, Suelen Santos; Alvarenga, Daniele Sapede; Panis, Carolina; Cataneo, Allan Henrique Depieri; Bordignon, Juliano; Silveira, Guilherme Ferreira; Yamauchi, Lucy Megumi; de Sá, Jussevania Pereira Santos Rubro; Felipe, Ionice; Pavanelli, Wander Rogério; Conchon-Costa, Ivete
• Is Part Of: Immunopharmacology and immunotoxicology. Volume 40:Number 5(2018)
• Page Start: 387
• Page End: 392
• Publication Date: 2018-09-03
• Subjects: Leishmania amazonensis -- Con-A -- PBMC -- cytokine -- reactive oxygen species -- iNOS; Immunopharmacology -- Periodicals; Immunotoxicology -- Periodicals; Antibody-toxin conjugates -- Periodicals; Immunology -- Periodicals; 615.37
• DOI: 10.1080/08923973.2018.1510960
• Languages: English
• ISSNs: 0892-3973

Abstract

Abstract: The context of the article: Leishmania amazonensis has a wide geographical distribution throughout South American countries and can cause self-healing to severe cases as mucocutaneous or visceral forms. Leishmaniasis presents a balance of inflammatory and anti-inflammatory cytokines which is responsible for promoting the activation of phagocytes, essential to control the infection and lead to tissue repair/resolution of the disease, respectively. Results and discussion: Our model revealed that the treatment with Con-A was capable to stimulate human PBMC cells by increasing the phagocytic capacity and promoting parasite elimination. The pretreatment with Con-A promoted inflammatory (IFN-γ, TNF-α, IL-2 and IL-6) and anti-inflammatory (IL-4 and IL-10) cytokines production, increased the reactive oxygen species (ROS) sinthesys as well as the expression and presence of iNOS enzyme, but not nitric oxide production. Conclusion: Based on the data obtained, it was possible to infer that Con-A induces the ROS production, responsible for eliminating parasites in addition to regulatory cytokines synthesis which are important for disease resolution.