Novel microsatellite markers for the endangered neotropical fish Brycon orbignyanus and cross-amplification in related species. Issue 4 (2nd October 2018)

• Record Type: Journal Article
• Authors: Souza, Felipe Pinheiro de; Urrea-Rojas, Angela Maria; Ruas, Claudete de Fátima; Povh, Jayme Aparecido; Ribeiro, Ricardo Pereira; Ruas, Eduardo Augusto; Giacomin, Renata Mussoi; Goes, Bruna Delgado; Castro, Pedro Luiz de; Lopera-Barrero, Nelson Mauricio
• Is Part Of: Italian journal of animal science. Volume 17:Issue 4(2018)
• Page Start: 916
• Page End: 920
• Publication Date: 2018-10-02
• Subjects: Genetic conservation -- genetic diversity -- molecular marker -- Piracanjuba -- SSR; Animal culture -- Periodicals; Livestock -- Italy -- Periodicals; Veterinary medicine -- Italy -- Periodicals; Animal culture; Livestock; Veterinary medicine; Italy; Periodicals; Periodicals; 636.005
• DOI: 10.1080/1828051X.2018.1436008
• Languages: English
• ISSNs: 1828-051X

Abstract

Abstract: Brycon orbignyanus is a neotropical fish found in several South American countries. This species has high ecological importance and is included in the list of fish threatened with extinction. In this study, we report the development and characterisation of 12 microsatellite primers of B. orbignyanus for future genetic studies. Screening of the 12 markers in a sample of 35 individuals identified seven polymorphic loci and 22 alleles, with one to three alleles per locus. The polymorphic information content ranged from 0.054 to 0.542, with four loci showing significant deviation from Hardy–Weinberg equilibrium. Two loci possessed null alleles. Cross-species amplification tests in two closely related species showed that 11 loci are transferrable to Brycon gouldingi ( B. gouldingi ) and nine are transferrable to B. falcatus. The microsatellite primers designed may be used for genetic variability and population structure studies in wild populations, fish farms and restocking programmes of B. orbignyanus and the two related species.