A DNA minimachine for selective and sensitive detection of DNA. Issue 2 (2nd January 2019)

• Record Type: Journal Article
• Authors: Lyalina, Tatiana A.; Goncharova, Ekaterina A.; Prokofeva, Nadezhda Y.; Voroshilina, Ekaterina S.; Kolpashchikov, Dmitry M.
• Is Part Of: Analyst. Volume 144:Issue 2(2019)
• Page Start: 416
• Page End: 420
• Publication Date: 2019-01-02
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c8an02274g
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : Synthetic molecular machines have been explored to manipulate matter at the molecular level. Abstract : Synthetic molecular machines have been explored to manipulate matter at the molecular level. Here, we designed a multifunctional DNA nano-construct, dubbed a 'DNA minimachine' (DMM), which (i) tightly binds complementary DNA; (ii) recognizes specific fragments with high selectivity and (iii) amplifies output signals. DMM1 detects lower concentrations of both single-stranded DNA and double-stranded DNA compared to a conventional probe. This study sets a direction towards the development of molecular machines for selective, sensitive and cost-efficient DNA analysis.