Α-Phellandrene enhances the immune response and resistance against Vibrio alginolyticus in white shrimp (Litopenaeus vannamei). Issue 84 (January 2019)

• Record Type: Journal Article
• Authors: Wu, Chih-Chung; Lin, Chia-Ling; Huang, Chun-Yung; Hsieh, Shuchen; Liu, Chun-Hung; Hsieh, Shu-Ling
• Is Part Of: Fish & shellfish immunology. Issue 84(2019)
• Page Start: 1108
• Page End: 1114
• Publication Date: 2019-01
• Subjects: α-Phellandrene -- Litopenaeus vannamei -- immune responses -- Vibrio alginolyticus; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2018.11.013
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Innate immunity and resistance against Vibrio alginolyticus in white shrimp, Litopenaeus vannamei, that received α-phellandrene were examined. The results indicated that the percent survival of shrimp receiving 4, 8, and 12 μg g −1 α-phellandrene was significantly higher than that of control shrimp after 72 h (p < 0.05). In a separate experiment, the phenoloxidase (PO), respiratory bursts, superoxide dismutase (SOD), and phagocytic and lysozyme activity of L. vannamei receiving 8 and 12 μg g −1 α-phellandrene were significantly higher than those of the other groups upon challenge with V. alginolyticus at 24–60, 36–60, 12–60, 12–72 and 48–72 h, respectively. However, no significant differences in the total haemocyte counts (THC) of L. vannamei receiving any dose of α-phellandrene and of control shrimp were observed at 12–72 h. The expression (mRNA transcripts) of the immune genes prophenoloxidase (proPO), LPS- and β-1, 3-glucan-binding protein (LGBP) and peroxinectin (PE) of shrimp receiving α-phellandrene at 8 and 12 μg g −1 significantly increased after challenge with V. alginolyticus for 72 h (p < 0.05). We conclude that the immune ability and resistance against V. alginolyticus infection increased in L. vannamei receiving >4 μg g −1 α-phellandrene. These results indicated that α-phellandrene plays an important role in the innate immunity of white shrimp. Highlights: α-phellandrene plays an important role in the innate immunity of white shrimp. α-phellandrene may have an effect on chemoprevention in shrimp. α-phellandrene can effectively enhance shrimp immunity and disease resistance against the pathogen V. alginolyticus through cellular and humoural immune responses.