Stress-immune responses and DNA protection function of thioredoxin domain containing 12 in zebrafish (Danio rerio). Issue 84 (January 2019)

• Record Type: Journal Article
• Authors: Kulatunga, D.C.M.; Dananjaya, S.H.S.; Nikapitiya, Chamilani; Godahewa, G.I.; Cho, Jongki; Kim, Cheol-Hee; Lee, Jehee; De Zoysa, Mahanama
• Is Part Of: Fish & shellfish immunology. Issue 84(2019)
• Page Start: 1030
• Page End: 1040
• Publication Date: 2019-01
• Subjects: Antioxidant -- Immune defense -- Oxidative stress -- Thioredoxin domain containing 12 -- Zebrafish (Danio rerio); Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2018.10.052
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Proteins with dithiol-disulfide oxidoreductase catalytic domain are well known for their capacity in the cellular redox homeostasis. In this study, we characterized the zebrafish thioredoxin domain containing 12 (Zf txndc12 ) gene, analyzed the transcriptional responses and studied the functional properties of its recombinant protein. Full-length cDNA of Zf txndc12 consists 519 bp coding region encoding 172 amino acids (AA) including the signal peptide. Highly consensus active motif ( 65 WCGAC 69 ) and probable ER retrieval motif ( 169 GDEL 172 ) were identified. Ubiquitous expression of Zf txndc1 2 mRNA was observed from one cell to juvenile stage as well as different organs of adult zebrafish. Moreover, whole mount in situ hybridization (WISH) results showed a higher expression of Zf txndc12 in primordial gills, central nerves system and eye. The tissue specific expression analysis (by qRT-PCR) also showed the highest expression in gills followed by brain in adult zebrafish. In larvae, up-regulated Zf txndc1 2 mRNA expression upon exposure to H2 O2, Edwardsiella tarda and Saprolegnia parasitica suggests that it may involve in both stress and immune responses. Moreover, transcriptional expression of Zf txndc12 was up-regulated upon Streptococcus iniae challenge in gills of adult zebrafish . The recombinant ZfTxndc12 (rZfTxndc12) was overexpressed, purified and tested for its biological activities. Results revealed that rZfTxndc12 is able to reduce the DNA damage and detoxify the H2 O2 toxicity in concentration dependent manner. Overall results suggest that Zf txndc12 is important antioxidant and immune functional member of the host defense system in zebrafish. Graphical abstract: Highlights: Txndc12 is important for oxidative stress defense and redox regulation. Constitutive expression of Zftxndc12 is ubiquitous in embryonic developmental stages and adult zebrafish. Immune challenge and oxidative stress induce the Zftxndc12 transcription in zebrafish larvae and adult's gills. >Recombinant Zftxndc12 shows in vitro ROS scavenging and cytoprotective activity.