An arabinogalactan isolated from Boswellia carterii: Purification, structural elucidation and macrophage stimulation via NF-κB and MAPK pathways. (January 2019)

• Record Type: Journal Article
• Authors: Bahramzadeh, Saman; Tabarsa, Mehdi; You, SangGuan; Yelithao, Khamphone; Klochkov, Vladimir; Ilfat, Rakhmatullin
• Is Part Of: Journal of functional foods. Volume 52(2019)
• Page Start: 450
• Page End: 458
• Publication Date: 2019-01
• Subjects: Boswellia carterii -- Arabinogalactan -- Immunomodulation -- NF-κB and MAPKs signaling pathways; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2018.11.025
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Water soluble acidic polysaccharides were isolated and purified from Boswellia carterii. The molecular weights of polysaccharides ranged from 295.5 to 504.7 × 10 3 g/mol. Polysaccharides exhibited notable RAW264.7 macrophage stimulating activity. Macrophages were activated through NF-κB and MAPK pathways via TLR4 and TLR2. Abstract: Polysaccharides from Boswellia carterii were isolated using hot water and fractionated on a DEAE Sepharose Fast Flow column to evaluate their structural characteristics and macrophage stimulating capacities. Crude and fractionated polysaccharides (BCF1 and BCF2 ) were mainly consisted of different amounts of neutral sugars (63.7–83.3%) and uronic acids (6.2–22.3%). The weight average molecular weights ( M w ) of polysaccharides greatly varied between 295.5 and 504.7 × 10 3 g/mol. The BCF2 was a highly active polysaccharide on RAW264.7 murine macrophage cells inducing considerable release of nitric oxide and proinflammatory cytokines including TNF-α, IL-1β and IL-6. The presence of p-NF-κB, p-JNK, p-ERK and p-p38 proteins in the cytoplasm of the stimulated macrophages indicated the activation of cells via NF-κB and MAPKs signaling pathways which was followed after CBF2 -macrophage interaction through TLR4 and TLR2 receptors. The most potent macrophage activating polysaccharide, BCF2, contained a backbone of (1 → 6)-β-galactopyranosyl units branched at C-3.