Characterization of four C1q/TNF-related proteins (CTRPs) from red-lip mullet (Liza haematocheila) and their transcriptional modulation in response to bacterial and pathogen-associated molecular pattern stimuli. Issue 84 (January 2019)

• Record Type: Journal Article
• Authors: Omeka, W.K.M.; Liyanage, D.S.; Priyathilaka, Thanthrige Thiunuwan; Kwon, Hyukjae; Lee, Seongdo; Lee, Jehee
• Is Part Of: Fish & shellfish immunology. Issue 84(2019)
• Page Start: 158
• Page End: 168
• Publication Date: 2019-01
• Subjects: CTRP -- Immune challenge -- Red-lip mullet -- Tissue-specific expression -- Transcriptional modulation; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2018.09.078
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The structural and evolutionary linkage between tumor necrosis factor (TNF) and the globular C1q (gC1q) domain defines the C1q and TNF-related proteins (CTRPs), which are involved in diverse functions such as immune defense, inflammation, apoptosis, autoimmunity, and cell differentiation. In this study, red-lip mullet ( Liza haematocheila ) CTRP4-like (MuCTRP4-like), CTRP5 (MuCTRP5), CTRP6 (MuCTRP6), and CTRP7 (MuCTRP7) were identified from the red-lip mullet transcriptome database and molecularly characterized. According to in silico analysis, coding sequences of MuCTRP4-like, MuCTRP5, MuCTRP6, and MuCTRP7 consisted of 1128, 753, 729, and 888 bp open reading frames (ORF), respectively and encoded 375, 250, 242, and 295 amino acids, respectively. All CTRPs possessed a putative C1q domain. Additionally, MuCTRP5, MuCTRP6, and MuCTRP7 consisted of a collagen region. Phylogenetic analysis exemplified that MuCTRPs were distinctly clustered with the respective CTRP orthologs. Tissue-specific expression analysis demonstrated that MuCTRP4-like was mostly expressed in the blood and intestine. Moreover, MuCTRP6 was highly expressed in the blood, whereas MuCTRP5 and MuCTRP7 were predominantly expressed in the muscle and stomach, respectively. According to the temporal expression in blood, all MuCTRPs exhibited significant modulations in response to polyinosinic:polycytidylic acid (poly I:C) and Lactococcus garvieae ( L. garvieae ). MuCTRP4-like, MuCTRP5, and MuCTRP6 showed significant upregulation in response to lipopolysaccharides (LPS). The results of this study suggest the potential involvement of Mullet CTRPs in post-immune responses. Highlights: CTRP4-like, CTRP5, CTRP6 and CTRP7 of Liza haematocheila were characterized. Globular C1Q and collagen domains were found. MuCTRP4-like, 5, 6 and 7 expresses ubiquitously in all the tissues examined. Immune challenged with LPS, poly I:C and L. garvieae.