Cerebellar Upregulation of Cell Surface Death Receptor–Mediated Apoptotic Factors in Harmaline-Induced Tremor: An Immunohistochemistry Study. (November 2018)

• Record Type: Journal Article
• Authors: Erekat, Nour S
• Is Part Of: Journal of cell death. Volume 11(2018)
• Publication Date: 2018-11
• Subjects: TNF-α -- active caspase-8 -- cerebellum -- Purkinje cells -- tremor -- harmaline; Cell death -- Periodicals; Cell Death; Cells; Cell Physiological Phenomena; Cell Biology; Cell death; Electronic journals; Periodicals; Fulltext; Internet Resources; Periodicals; Periodicals; 571.936
• DOI: 10.1177/1179066018809091
• Languages: English
• ISSNs: 1179-0660

Abstract

Active caspase-3-mediated apoptosis has been implicated in the pathogenesis of harmaline-induced tremor. The aim of this study is to illustrate the impact of tremor induction on the expression of factors mediating the cell surface death receptor–dependent apoptosis. A total of 20 normal Wistar rats were randomly selected and equally divided into control and experimental groups. Tremor was induced in the experimental group by injecting the rats with a single dose of harmaline (50 mg/kg). After that, cerebellar tissues were evaluated by immunohistochemistry to examine the expression of tumor necrosis factor α (TNF-α) and active caspase-8 in the 2 groups of animals. TNF-α and active caspase-8 expression was significantly higher in cerebella from experimental rats compared with that in those from the control rats ( P value < .01). Thus, our present data suggest the association of tremor induction with the cerebellar overexpression of TNF-α and active caspase-8, correlative with Purkinje cell (PC) loss indicated by loss of calbindin immunoreactivity, indicating the induction of the cell surface death receptor–mediated apoptosis.