Reactive astrocytes in multiple sclerosis impair neuronal outgrowth through TRPM7‐mediated chondroitin sulfate proteoglycan production. Issue 1 (19th November 2018)

• Record Type: Journal Article
• Authors: Kamermans, Alwin; Planting, Kirsten E.; Jalink, Kees; van Horssen, Jack; de Vries, Helga E.
• Is Part Of: Glia. Volume 67:Issue 1(2019)
• Page Start: 68
• Page End: 77
• Publication Date: 2018-11-19
• Subjects: astrocytes -- chondroitin sulfate proteoglycan -- multiple sclerosis -- neurodegeneration -- TRPM7; Neuroglia -- Periodicals; Neurology -- Periodicals; 611.0188
• DOI: 10.1002/glia.23526
• Languages: English
• ISSNs: 0894-1491

Abstract

Abstract: Multiple sclerosis (MS) is a chronic inflammatory disorder of the central nervous system (CNS), characterized by inflammation‐mediated demyelination, axonal injury and neurodegeneration. The mechanisms underlying impaired neuronal function are not fully understood, but evidence is accumulating that the presence of the gliotic scar produced by reactive astrocytes play a critical role in these detrimental processes. Here, we identified astrocytic Transient Receptor Potential cation channel, subfamily M, member 7 (TRPM7), a Ca 2+ ‐permeable nonselective cation channel, as a novel player in the formation of a gliotic scar. TRPM7 was found to be highly expressed in reactive astrocytes within well‐characterized MS lesions and upregulated in primary astrocytes under chronic inflammatory conditions. TRPM7 overexpressing astrocytes impaired neuronal outgrowth in vitro by increasing the production of chondroitin sulfate proteoglycans, a key component of the gliotic scar. These findings indicate that astrocytic TRPM7 is a critical regulator of the formation of a gliotic scar and provide a novel mechanism by which reactive astrocytes affect neuronal outgrowth. Main Points: TRPM7 is highly expressed in reactive astrocytes within MS lesions. Enhanced astrocytic TRPM7 levels impair neurite outgrowth by increased production of CSPGs, a key component of the gliotic scar.