Genetic rescue in a plant polyploid complex: Case study on the importance of genetic and trait data for conservation management. Issue 10 (25th April 2018)

• Record Type: Journal Article
• Authors: Schmidt‐Lebuhn, Alexander N.; Marshall, David J.; Dreis, Brad; Young, Andrew G.
• Is Part Of: Ecology and evolution. Volume 8:Issue 10(2018)
• Page Start: 5153
• Page End: 5163
• Publication Date: 2018-04-25
• Subjects: polyploidy -- Rutidosis lanata -- self‐incompatibility; Ecology -- Periodicals; Evolution -- Periodicals; 577.05
• DOI: 10.1002/ece3.4039
• Languages: English
• ISSNs: 2045-7758

Abstract

Abstract: Knowledge of the biology of rare plant species is indispensable to aid their survival and to inform efficient conservation actions, but in many cases relevant data are lacking. In addition, while studies of conservation genetics have provided a wealth of information on the considerations arising from inbreeding, mate limitation, or local adaptation, the impact of intraspecific polyploidy remains understudied. In this study, we examined the breeding system of the rare Australian daisy Rutidosis lanata (Asteraceae) and screened ten of its populations for their ploidy level to develop recommendations for management actions, in particular, with regard to seed sourcing and genetic rescue. We found R. lanata to represent a polyploid complex, with tetraploid, pentaploid and hexaploid individuals coexisting in the same species. Crossing experiments confirmed R. lanata to be self‐incompatible. Mate availability varied from c. 49% to c. 76% across populations. Most populations showed mate availability of c. 50%–70%, suggesting that mate limitation resulting from a lack of local genetic diversity may cause or at least contribute to reduced seed set. Crossing between populations resulted in significantly higher reproductive success for all populations except one, suggesting the possibility of genetic rescue through population mixing. However, the crossing experiments also showed that pentaploids suffer from a severely reduced paternal reproductive fitness. Any additional hybrids between tetraploids and pentaploids, as would be created by mixing populations with different genome copy numbers during conservation work, would consequently exacerbate mate limitation and thus reduce population viability. We conclude that seed set and thus population viability can be maximized by mixing populations with the same number of genome copies, but that populations with different numbers should be kept spatially separated. The case of Rutidosis lanata provides an example and a potential template for examining the conservation genetics of other species that may constitute polyploid complexes. Abstract : The rare southern Queensland endemic daisy Rutidosis lanata is demonstrated to be self‐incompatible and to constitute a polyploid complex. There is evidence of mate limitation and improved seed set after crossing between populations, but crosses between ploidy levels suffer from reduced fertility. Conservation actions will ideally be based on an understanding of the geographic distribution of ploidy levels.