Liquid Metal‐Based Multifunctional Micropipette for 4D Single Cell Manipulation. Issue 7 (3rd May 2018)

• Record Type: Journal Article
• Authors: Chow, Yu Ting; Man, Tianxing; Acosta‐Vélez, Giovanny F.; Zhu, Xiongfeng; Wen, Ximiao; Chung, Pei‐Shan; Liu, Tingyi "Leo"; Wu, Benjamin M.; Chiou, Pei‐Yu
• Is Part Of: Advanced science. Volume 5:Issue 7(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-05-03
• Subjects: cell manipulation -- dielectrophoresis -- electrorotation -- liquid metals; Science -- Periodicals; 505
• DOI: 10.1002/advs.201700711
• Languages: English
• ISSNs: 2198-3844

Abstract

Abstract: A novel manufacturing approach to fabricate liquid metal‐based, multifunctional microcapillary pipettes able to provide electrodes with high electrical conductivity for high‐frequency electrical stimulation and measurement is proposed. 4D single cell manipulation is realized by applying multifrequency, multiamplitude, and multiphase electrical signals to the microelectrodes near the pipette tip to create 3D dielectrophoretic trap and 1D electrorotation, simultaneously. Functions such as single cell trapping, patterning, transfer, and rotation are accomplished. Cell viability and multiday proliferation characterization has confirmed the biocompatibility of this approach. This is a simple, low‐cost, and fast fabrication process that requires no cleanroom and photolithography step to manufacture 3D microelectrodes and microchannels for easy access to a wide user base for broad applications. Abstract : A liquid metal‐based multifunctional microcapillary pipette that is able to provide high‐conductivity electrodes is presented. It allows high‐frequency electrical stimulation and measurement. 4D single cell manipulation is achieved using a multifunctional pipette by supplying multifrequency, multiamplitude, and multiphase electrical signals in the megahertz range to create 3D dielectrophoresis trap and 1D electrorotation, simultaneously.